Naser Gilani; Mehmat Ozaslan; Rozhgar A.Khailany
Abstract
Vitamin D (Vit D) ,as an antioxidant contributes to a wide range of diseases including obesity, type 2 diabetes, multiple sclerosis, and certain cancers that oxidative stress plays ...
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Vitamin D (Vit D) ,as an antioxidant contributes to a wide range of diseases including obesity, type 2 diabetes, multiple sclerosis, and certain cancers that oxidative stress plays a vital role in their development. Excessive oxidative stress can damage to DNA and nucleotide pool. Base excision repair and house-cleaning enzymes can protect genome so that any disruption in expression of these genes indicates enhanced susceptibility risk for diseases like cancer. The present study was conducted aimed at evaluating the effect of Vit D on the expression of MYH and MTH1 as DNA repair genes, as well as effect of ViD treatment in Human Umbilical Vein Endothelial Cells (HUVEC) cell line. To do this, bioinformatics tools were used to predict the interaction of MTH1 and MYH with VDR as a specific transcription factor (TF) for Vit D. The cell line was treated with VitD. Next, viability was evaluated using MTT assay. The mRNA expression of MTH1 and MYH was assessed using real-time PCR at 48h post-treatment with Vit D. Results of the study revealed that Vit D could regulate MTH1 and MYH transcript expression directly through its specific TF; VDR. In response to VitD treatment a different alteration was observed in DNA repair, and non-canonical nucleotide repair genes. Findings of this study showed a new regulation of DNA repair genes in Vit D signaling pathway, and it may be a new perspective for the therapeutic effect of Vit D on related diseases. Variation in interested genes may affect the vitD signaling and personalized medicine should be considered.
