@article { author = {Saremi, Mohammad Ali}, title = {Evaluation of Common Mutations in Exon 2 and 3 of the K-ras Gene in Patients with Lung Cancer}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {1-3}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01031}, abstract = {Lung cancer is the deadliest cancer in Iran after gastric cancer. The vast majority (85%) of cases of lung cancer are due to long-term tobacco smoking. About 10–15% of cases occur in people who have never smoked. These cases are often caused by a combination of genetic and environmental factors. Many human cancers are the result of mutations in the RAS family, and lung cancer is no exception. In this study, mutations in codon 12 and 13 of exon two were performed in 50 lung tumors from the Iranian Institute of Oncology. The exon 2 of the gene was amplified by PCR and sequenced for detection of the point mutation in codon 12 and 13. Of the 50 samples, 13 had mutations in codon 12 and 13, of which only two patients had single mutations in codon 12. No significant relationship was not found between age (P = 0.43) and gender (P = 0.37) and mutations in this gene. No significant relationship was found between disease stage and mutation in this gene (P = 0.51). Identifying k-ras gene mutations as an oncogene and having an effect on the treatment process can help the physician to choose the appropriate treatment.}, keywords = {Lung Carcinoma K,ras Gene Point Mutation DNA Sequencing}, url = {https://www.pmjournal.ir/article_39035.html}, eprint = {https://www.pmjournal.ir/article_39035_bcfa6416b4380772ad7217e6b11d58c8.pdf} } @article { author = {Esfahani, Vahid Reza and Saremi, Mohammad Ali}, title = {Investigation of the Relationship between PDYN Gene Polymorphisms and Tendency to Heroin Use}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {4-6}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01032}, abstract = {Numerous studies have been conducted to investigate the relationship between genetic factors and drug use tendency, many of which have shown a significant correlation between genetic factors and drug use, especially heroin and cocaine. The most important site of drug action is the brain, which contains a variety of nerve receptors. Dynorphins are opioid peptides derived from the prodynorphins precursor (PDYN). These opioid peptides are capable of binding to the three types of opioid receptors. Studies have shown that heroin and cocaine use is associated with increased PDYN gene expression in specific areas of the brain. In this study, we investigated the association between rs910080 polymorphism in the 3'UTR region and the number of VNTR sequences in the promoter region with the tendency of heroin use in 155heroin addicts and 150 control with RFLP method. Results showed a significant relationship between heroin abuse and CC genotype in rs910080 polymorphism (P = 0.001), but no significant relationship between VNTR promoter repeats and heroin abuse. Rs910080 polymorphism can be used as a distinguishing factor.}, keywords = {Heroin Addicts,gene polymorphism,Prodynorphins,RFLP,VNTR}, url = {https://www.pmjournal.ir/article_39036.html}, eprint = {https://www.pmjournal.ir/article_39036_c325eff6648915308edcbe34e59b3fea.pdf} } @article { author = {Alikarami, Mahsa}, title = {The Effect of Kombucha Tea on Oxidative Stress Parameters in Mice Treated with Silver Nanoparticles}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {7-9}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01033}, abstract = {The use of silver as an antibacterial goes back to ancient times. Adding a small number of silver nanoparticles to different surfaces can cause the antimicrobial coating. Use at high doses has toxic effects, which can alter cell morphology, increase oxidative stress, increase membrane permeability, decrease cell growth, and ultimately cause cell death through apoptosis or necrosis. In this study, we evaluated the antioxidant effect of kombucha beverage in mice treated with silver nanoparticles. The amount of SOD, CAT, MAD, and GST enzymes were evaluated in four groups include control groups, silver nanoparticles treated, Cambodian beverage treatment, and Nano + kombucha treated. The results showed an increase in oxidative stress after treatment with silver nanoparticles. It has also demonstrated that kombucha drinks can have a protective role and reduce the oxidative stress induced by silver nanoparticles. It is therefore suggested that it can be used as a potent antioxidant against silver nanoparticles.}, keywords = {Silver Nanoparticle,Stress oxidative,Kombucha,Antioxidan}, url = {https://www.pmjournal.ir/article_39037.html}, eprint = {https://www.pmjournal.ir/article_39037_9e24d2d3ac17c9bfb295d65915e76fc3.pdf} } @article { author = {Poorhasan, Nafise}, title = {The Role of Curcumin Supplementation on the Side Effects of Busulfan and the Process of Apoptosis}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {10-12}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01034}, abstract = {One of the most well-known side effects of anticancer drugs is the disruption of spermatogenesis. One of the most important causes of infertility in men is impaired spermatogenesis. Busulfan is a cytotoxic alkylating agent and belongs to the alkyl sulfonate group. This agent causes DNA damage by cross-linking with DNA. It kills spermatogonial stem cells. The use of healthy antioxidants can reduce the side effects of alkylating agents. In this study, we measured the protective effect of curcumin on testicular tissue of mice treated with busulfan by measuring BAX expression as an apoptotic inducer and Bcl2 as an apoptotic inhibitor. The expression levels of the two apoptotic regulatory genes Bax and Bcl-2 were evaluated by realtime PCR in four groups of mice (control, treatment with busulfan, treatment with curcumin, and treatment with busulfan + curcumin). It was observed in the busulfan group that bcl2 expression decreased, and Bax increased significantly, and the busulfan + curcumin group showed the opposite effect. Curcumin can be beneficial as a dietary supplement in cancer patients treated with drugs such as busulfan.}, keywords = {Cancer,Busulfan,curcumin,Antioxidant,Apoptosis}, url = {https://www.pmjournal.ir/article_39038.html}, eprint = {https://www.pmjournal.ir/article_39038_de518c4183b20d05a1d32c0744103014.pdf} } @article { author = {Houshmand, Massoud and Saadat, Seyed Hassan}, title = {Association of Glutathione S-Transferase A1 Gene Polymorphism-69 Promoter with Colorectal Cancer}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {13-15}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01035}, abstract = {Colorectal cancer is the third most common cancer and the second leading cause of cancer deaths in the world. Risk factors such as obesity, low-fiber diet, sedentary, and alcohol have been implicated in the development of this cancer. Cells have many mechanisms in place to fight malignancy and cancer, one of which is the detoxification system that protect the cell against DNA damage. One of the essential components of this system is the glutathione s-transferase. In this study, the genotype of region -69 promoter of this gene in 150 colorectal cancer patients and 150 healthy people was investigated by the RFLP method. T/T genotype is significantly associated with colorectal cancer (P = 0.0001), C/T genotype did not show a significant association with colorectal cancer and its frequency was not significantly different in both healthy and patient groups (P = 0.074), Most genotypes in the two groups were homozygous C / C and did not show a significant association with colorectal cancer.}, keywords = {Colorectal Cancer Glutathione s,transferase RFLP Polymorphism}, url = {https://www.pmjournal.ir/article_39039.html}, eprint = {https://www.pmjournal.ir/article_39039_73187d1f3f0722a7064d20163c142108.pdf} } @article { author = {Javanbakht, Mohammad}, title = {Frequency of Human Papillomavirus Types in Women with Genital Warts by Probe-based Real-time PCR}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {16-18}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01036}, abstract = {The second most common cancer and the fourth leading cause of death in women is cervical cancer. In 90% of cases of human papillomavirus infections, HPV is the cause of this cancer. More than 200 different types of the virus have been identified, including low-risk and high-risk variants. According to statistics, infections from types 16 and 18 of the virus account for 70% of cervical cancers. This cancer is preventable, and one of the ways to prevent it is to have regular screenings. Probe-based real-time PCR methods are more accurate and sensitive methods for detection. In this study, we performed HPV typing with probed base real-time PCR in 50 genital wart samples and checking the relationship between questionnaire information.}, keywords = {Cervical Cancer HPV Real,time PCR Screening}, url = {https://www.pmjournal.ir/article_39040.html}, eprint = {https://www.pmjournal.ir/article_39040_529ee72ac7f214d268780282edaf22ee.pdf} } @article { author = {Mohammadi Pour, Fatemeh and Imani Farahani, Faezeh}, title = {Association between CDKAL1 Gene Polymorphism and Risk of Type 2 Diabetes in Population of Lorestan Province}, journal = {Personalized Medicine Journal}, volume = {4}, number = {15}, pages = {19-21}, year = {2019}, publisher = {AmitisGen TECH Dev Group}, issn = {2476-5538}, eissn = {2717-3860}, doi = {10.21859/pmj01037}, abstract = {Diabetes Mellitus is defined as a metabolic disorder characterized by chronic hyperglycemia. The more prevalent form, type 2 diabetes, usually begins as insulin resistance, a disorder in which the cells do not use insulin properly. T2DM is a complex multifactorial disease in which multiple genetic variants interact with environmental factors to trigger the disease. There is sufficient evidence that T2DM has a strong genetic basis. One of the identified candidate genes associated with type 2 diabetes is CDKAL1, reduced expression of CDKAL1 would result in enhanced activity of CDK5 in β cells, which would lead to decreased insulin secretion. In this study, the association of polymorphism rs10946398 of CDKAL1 with type 2 diabetes was evaluated in 200 people by RFLP-PCR method. No significant association was found between the genotypes of polymorphism rs10946398 and type 2 diabetes in the target population. It is suggested that this polymorphism association with type 2 diabetes in larger populations evaluated.}, keywords = {Diabetes,gene polymorphism,T2DM,CDKAL1,RFLP}, url = {https://www.pmjournal.ir/article_39041.html}, eprint = {https://www.pmjournal.ir/article_39041_85ef5219ed892f94f3fd1c595f79c816.pdf} }