AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
Association between rs362746 polymorphism of RELN and Schizophrenia in Iranian Patients
1
3
EN
Shima
Alimohammadi
Department of microbiology, karaj branch, Islamic Azad University, karaj, Iran
sh1390am@gmail.com
Fateme
Frootan
Maya Milk, Inc, Delaware, USA
10.22034/pmj.2021.244727
Genetic studies, there is a potential association of RELN with some psychological<br />disorders such as Autism Spectrum Disorders (ASD) schizophrenia (SCZ). The RELN<br />gene is located on chromosome 7q22.1 and encodes a large secretory protein of the<br />extracellular matrix (Reelin). In the present case-control study, we intended to investigate<br />the relationship between the rs362746 polymorphism of RELN and schizophrenia in a<br />group of schizophrenic and healthy subjects from northeastern Iran.30 unrelated schizophrenic patients and 30 matched control subjects were recruited The samples<br />from the participants underwent PCR and sequencing for RELN genotype identification.he genotype distribution for both study and control groups were not in Hardy–Weinberg equilibrium(P>0.05).However, it was found that the prevalence of<br />rs362746 polymorphism was significantly different between the groups. the present study supported the evidence that rs362746 polymorphism of RELN was a<br />genetic factor for schizophrenia susceptibility. However, there is a need for replication<br />studies on different populations and further investigations on the sex-specific association<br />of this gene with schizophrenia.
Schizophrenia,Reelin gene,single nucleotide polymorphism,Sequencing
https://www.pmjournal.ir/article_244727.html
https://www.pmjournal.ir/article_244727_d7646133fe618ba512c39af38308829d.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
A relationship between two polymorphisms (rs2660 and rs1800450) and coronavirus (COVID-19) in Iranian population
4
6
EN
Zahra
Sadeghi
Department of genetic, Tehran-North branch, Islamic azad university, Tehran,
Iran
z_sadeghi@email.com
Hossein
Pakzad
Department of biology, Tehran-east branch, Islamic azad university, Tehran,
Iran
Massoud
Houshmand
Department of Medical Genetics, National Institute for Genetic Engineering
and Biotechnology (NIGEB), Tehran, Iran
housh62@yahoo.com
10.22034/pmj.2021.244728
Warning of the World Health Organization (WHO) indicates that novel coronavirus (COVID-19) is pandemic and causes global concern. COVID-19 has acute respiratory symptoms which leads to die in many cases through the world. We have found seven variants in 300 patients based on Next Generation Sequencing (NGS) which are related to infectious disease. According to the databases, we confirmed that rs2660 and rs1800450 have association with COVID-19 in the Iranian population.
COVID-19,Infection,NGS,Polymorphism
https://www.pmjournal.ir/article_244728.html
https://www.pmjournal.ir/article_244728_9fa0045b49906ec61c85af426dad2633.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
Investigating the causes of antibiotic resistance in bacteria
7
11
EN
AmirHossein
Akbari Aghababa
Department of Micrbiology, Faculty of biology, Islamic Azad University, North Branch,Tehran, Iran
amirhosseinakbari22@gmail.com
Mona
Nadi
Department of Cell and Molecular Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
10.22034/pmj.2021.244729
The discovery and production of (synthetic) antibiotics in the first half of the previous century has been one of medicine’s greatest achievements. The use of antimicrobial agents has reduced morbidity and mortality of humans and contributed substantially to human’s increased life span. The issue of antibiotic resistance has received considerable attention due to the problem of the emergence and rapid expansion of antibiotic-resistant pathogenic bacteria. Antibiotic resistance exhibited by bacteria can be intrinsic, acquired, or adaptive. Therefore, efforts to develop antibiotics and study mechanisms of resistance should be continuous, resilient and steady. In the following sections, we will focus on the molecular and biochemical mechanisms of bacterial resistance, illustrating specific situations that are often encountered in clinical practice.
Antibiotic resistance,Bacteria,Horizontal Gene Transfer,Efflux pumps
https://www.pmjournal.ir/article_244729.html
https://www.pmjournal.ir/article_244729_f5c487cdfd822b31dbd1aad493e710e1.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
Epithelial-mesenchymal transition in breast cancer
12
16
EN
Naser
Gilani
Department of Biology, Gaziantep University, Gaziantep, Turkey
Farabi Molecular Laboratory, Irbil, Iraq
farabilaboratory@yahoo.com
Mehmet
Ozaslan
Department of Biology, Gaziantep University, Gaziantep, Turkey
10.22034/pmj.2021.244731
One of the key molecular mechanisms contributing to the metastatic progression is epithelial to mesenchymal transition (EMT), which drives invasion and migration of various cancer including breast cancer.During tumorigenesis, changes in EMT regulatory pathways lead to a loss of cellular adhesions, changes in the polarization of the cell and cytoskeleton, detachment, migration, intra-vasation, and survival in the vascular system; extravasation, and finally, metastasis.EMT is largely mediated by a core set of EMT-activating transcription factors. The master regulators of the EMT include many pathways, however the primary mediators of the EMT include signaling through TGF-, Notch and Wnt. The role of EMT in breast cancer has been demonstrated via numerous in vitro studies in normal and malignant mammary epithelial cells and via in vivo studies using mouse models of breast cancers. Studying the regulatory pathways of the EMT process can be used as a tool for cancer monitoring ,treatment and possible direct targets for new-combination anticancer personalized medicine.
Epithelial to mesenchymal transition,Breast cancer,Cell Signaling,Personalized Medicine
https://www.pmjournal.ir/article_244731.html
https://www.pmjournal.ir/article_244731_e9071081de474040fced4dd0ee02639e.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
Covid19 disease and the effect of genetic factors on its severity and weakness
17
22
EN
Abbas
Ghasemzadeh
ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR,Tehran, Iran
ghasemzadeh313@gmail.com
Vahidreza
Esfahani
Department of Cellular and Molecular Tehran Medical Sciences Branch,lslamic Azad University, Tehran, Iran
10.22034/pmj.2021.244730
Severe Acute Respiratory Syndrome<br />Coronavirus 2 (SARS-CoV-2), is a severe infection with respiratory and systemic<br />manifestations. This infectious disease has a complex course and manifests itself with<br />various clinical presentations, ranging from asymptomatic infection to a severe clinical<br />course. These variations in severity have raised the question of whether the genetic or<br />epigenetic variations have a role in COVID-19 susceptibility or severity, and that these<br />factors can be used to predict the disease course. A whole-genome sequencing performed<br />on 95 samples of SARS-CoV-2 identified 116 unique mutations, most of which were<br />missense and synonymous. Moreover, some studies have reported a relationship between<br />the COVID-19 severity and the genes ACE and TMPRSS2. The present review provides<br />an overview of different genes that have been found to be implicated or related to the<br />susceptibility to COVID-19 or its severity.
Covid19,ACE gene,TMPRSS2 gene,Infection
https://www.pmjournal.ir/article_244730.html
https://www.pmjournal.ir/article_244730_31b7d1361df9f341d51df05f9845d346.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
6
21
2021
06
20
The application of DNA-conjugated gold nanoparticles to detect metabolites and nucleic acids in personalized medicine
23
25
EN
Roya
Amirinejad
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Zeinab
Shirvani-Farsani
Department of Cell and Molecular Biology, Faculty of Biological Sciences and Technology, Shahid Beheshti University G.C., Tehran, Iran
Sohameh
Mohebbi
Department of Biotechnology, Faculty of Basic Science, Ale-Taha Institute of Higher Education, Tehran, Iran.
s.mohebbi@aletaha.ac.ir
10.22034/pmj.2021.244733
In this article, the features of DNA-functionalized goldnanoparticles (GNRs), including the size-dependent color, the amount of conjugated DNA, and the fluorescence quenchers will be described. DNA and aptamer conjugated GNRs can be applied for producing the colorimetric and fluorescent biosensors to detect all types of disease markers including DNA, RNA, protein and other small molecular metabolites.<br />The early phase of this work is performed in clean buffers and serum samples. DNA-conjugated GNRs delivery into the cells is recently used for intracellular diagnosis in personalized medicine. Simultaneously, DNA-functionalized GNRs can be used to deliver the antisense DNA for gene therapy applications.<br />With targeting both diagnosis and treatment applications, DNA- functionalized GNRs can be used as a suitable approach to reach theranostics purposes (diagnosis and treatment in a simultaneous manner).
GNRs,Aptamers,Biosensors,Fluorescence,Metabolites,Personalized Medicine
https://www.pmjournal.ir/article_244733.html
https://www.pmjournal.ir/article_244733_088ba15a1b3e392b8849ead98e645ae6.pdf