AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Analysis of EGFR gene mutations in tissue samples of lung cancer tumors
1
4
EN
Blnd
Ibrahim Mohammed
Biology Department, College of Science, Salahaddin University-Erbil, Erbil, Iraq
blnd.mohammed@su.edu.krd
Amir
Mohammadi
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran
Nafise
Poorhasan
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
nafis.p1993@gmail.com
10.22034/pmj.2020.43451
Lung cancer is the leading cause of cancer deaths worldwide. Approximately 25% of nonsmall-cell lung cancers have mutations in the<em> EGFR </em>gene, most of which occur in hotspot regions in exons 18, 19, 20, and 21. In-frame deletions in exon 19 (~50%) and the L858R point mutation in exon 21 (~40%) are associated with a favorable response to EGFR tyrosine kinase inhibitors. In this study, mutations of two exons of 19 and 21 in 50 lung cancer tumor samples were investigated by the sequence method. From 50 lung cancer patients, 8 (16%) patients had an L858R (c.2573T>G) mutation, 6 (12%) patients had deletion type 1a mutation, and one patient had deletion type 1b mutation. Examining the sequence of candidate genes associated with lung cancer can be very important in choosing the right treatment approach.
Lung cancer,EGFR gene,Sequencing,Drug resistance,TKI
https://www.pmjournal.ir/article_43451.html
https://www.pmjournal.ir/article_43451_3b7ae99e366ff787db6293d16a55b0ae.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Comparison of different methods of DNA extraction from paraffin-embedded tissues
5
8
EN
Ramadhan
Ibrahim
Fish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University, Erbil, Iraq
Saeed
Megdadi
Department of biology, Nourdanesh institute of higher education, Meymeh, Isfahan, Iran
megdadi1992@gmail.com
Sareh
Bakhshandeh bavarsad
Department of Public Management, Faculty of Management, University of Shahrekord, Iran
Najme
Shojaei
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
10.22034/pmj.2020.43452
The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method.
paraffin-embedded tissues,DNA extraction,salting-out,phenol-chloroform
https://www.pmjournal.ir/article_43452.html
https://www.pmjournal.ir/article_43452_f4334212cbd8e556ab18d048f5e9609f.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis
9
11
EN
Fawziah
M. Mohammed
Faculty of Allied Health Sciences KuwaitnMedical Laboratory Sciences
fawziahmam@hsc.edu.kw
Hossein
Pakzad
Department of biology, Tehran-east branch, Islamic azad university, Tehran, Iran
Vahid Reza
Esfahani
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
vahidstar26@gmail.com
10.22034/pmj.2020.43453
Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.
Myeloproliferative,JAK2,point mutation,High-Resolution Melting
https://www.pmjournal.ir/article_43453.html
https://www.pmjournal.ir/article_43453_696926aea6f2fdb675a0c9c98112c933.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Increased expression of the lnc H19 gene in the plasma of people with breast cancer
12
14
EN
kazhaleh
Mohammadi
Researcher and lecture assistant at the Medical Laboratory Science Department, Knowledge University, Kurdistan Region, Erbil, Iraq
kazhaleh.mohammadi@knu.edu.iq
Sadegh
Shojaei Baghini
Plant Biotechnology, Agriculture Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran
shojai_sadegh88@yahoo.com
Mohammad Ali
Saremi
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
masaremi@yahoo.com
10.22034/pmj.2020.43454
The majority of ncRNAs are known as long non-coding RNAs (lncRNAs) whose length exceeds 200 nucleotides. H19, a lncRNA, is the transcription product of the H19 gene, an oncogene in breast cancer, and is highly expressed in cancer tissues compared with normal tissues. The expression level of H19 is associated with the oncogenesis, proliferation, invasion, metastasis, and drug resistance of breast cancer. H19 expression levels were detected in breast cancer plasma using qRT-Real-Time PCR assay in 50 breast cancer samples and 50 healthy control samples. The results showed that the expression of this gene in both the tissue and the plasma of patients increased compared to that of healthy individuals.
lncRNAs,Breast cancer,Gene expression,Plasma,qRT-Real-Time PCR
https://www.pmjournal.ir/article_43454.html
https://www.pmjournal.ir/article_43454_4a6cbd9219c6fd9f7ae988955f8df29e.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
The effect of silver nanoparticles on MCF7 breast cancer cell
15
17
EN
Abdulkarim
Karim
College of Science, SALAHADDIN UNIVERSITY-ERBIL (SUE)
abdulkarim.karim@su.edu.krd
Mohammad Ali
Keshavarz Shahbaz
Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran
ali.keshavarz1989@gmail.com
Afarin
Komam
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
10.22034/pmj.2020.43455
Currently, little is known about the mechanism(s) of AgNP-induced toxicity. Many previous studies, however, have provided strong evidence for a link between the AgNP-mediated production of ROS and the subsequent generation of oxidative stress. In the current study, the effects of Ag nanoparticles on the MCF-7 breast cell line were examined, and the biomarkers related to stress oxidative including GSH, superoxide dismutase, catalase, and ROS generation were evaluated. The results showed that Ag nanoparticles induced intracellular ROS generation in a dose- and time-dependent manner. Therefore, various studies should be performed to investigate the toxic effects of this substance on different cells.
Ag nanoparticle,MCF-7 cell line,Stress oxidative,Ros
https://www.pmjournal.ir/article_43455.html
https://www.pmjournal.ir/article_43455_b8b4e4ceea546eb4eefe420c7731b7c7.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Relationship between PAI1 promoter 4G/5g polymorphism and stroke
18
20
EN
Sahar
Hassannejad
Researcher and lecture assistant at the Medical Laboratory Science Department, Knowledge University, Kurdistan Region, Erbil, Iraq
sahar.najad@knowledge.edu.krd
Ehsan
Razeghian
Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran
Najme
Shojaei
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
10.22034/pmj.2020.43456
PAI-1 has become recognized as a central molecule linking pathogenesis and progression of thrombotic vascular events, including stroke. Clinical and experimental studies show that PAI-1 deficiencies cause accelerated fibrinolysis and bleeding, whereas elevated PAI-1 plasma levels are associated with vascular thrombosis. Raised PAI1 plasma levels are related to a 1-bp guanine deletion/insertion (4G/5G) polymorphism in the promoter of the PAI1 gene. The 4G allele is associated with higher plasma PAI1 transcription and activity. In the current study, the association of higher PAI-1 plasma levels and the prevalence of the 4G/5G polymorphism in the PAI-1 gene promoter region in young patients with stroke were explored. Significantly, higher PAI-1 levels were observed in patients when compared to controls (<em>p </em>=002). The 4G/5G polymorphisms were significantly associated with increased PAI-1 levels, with the variant homozygous 4G/4G corresponding to mean values in patients versus controls.
Stroke,PAI-1,Polymorphism,Plasma
https://www.pmjournal.ir/article_43456.html
https://www.pmjournal.ir/article_43456_aede7816e139e7092a1cb533144f578b.pdf
AmitisGen TECH Dev Group
Personalized Medicine Journal
2476-5538
2717-3860
5
17
2020
06
01
Investigating the relationship between VEGF gene C936T-rs3025039 polymorphism and type 2 diabetes
21
24
EN
Hadi
Yari
Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran.
yari.hadi@gmail.com
Niloofar
JAHANGIR SOOLTANI
Department of Medical
Biotechnology, National Institute
of Genetic Engineering and
Biotechnology, Tehran, Iran.
Mohammad Ali
Saremi
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
masaremi@yahoo.com
10.22034/pmj.2020.43457
Type 2 diabetes (T2D), formerly known as adult-onset diabetes, is characterized by high blood sugar, insulin resistance, and relative lack of insulin.Diabetic retinopathy, a secondary microvascular complication of diabetes mellitus, is the leading cause of blindness. There is extensive evidence that the pathologic ocular angiogenesis in diabetic retinopathy is regulated by the vascular endothelial growth factor-A (VEGF-A).The single nucleotide polymorphism (SNP) C936T (rs3025039) of the VEGF gene has been investigated in relation to cancer, endometriosis, and age-related macular degeneration. The relationship between the rs3025039 VEGF gene polymorphism and the risk of type 2 diabetic retinopathy in 80 DT2 patients was examined. No significant association was found between polymorphism C936T and type 2 diabetes. It is recommended that this study be repeated on a larger population.
type 2 diabetes,Diabetic retinopathy,single nucleotide polymorphism,microvascular
https://www.pmjournal.ir/article_43457.html
https://www.pmjournal.ir/article_43457_ac7f4367c28e6823ab33c2e079041962.pdf