TY - JOUR ID - 698498 TI - VitminD Treatment Change MTH1 and MYH Genes Expression in HUVEK cell JO - Personalized Medicine Journal JA - PMJ LA - en SN - 2476-5538 AU - Gilani, Naser AU - Ozaslan, Mehmat AU - A.Khailany, Rozhgar AD - Department of Biology, Gaziantep University, Gaziantep, Turkey. Farabi Molecular Laboratory, Irbil, Irag AD - Farabi Molecular Laboratory, Irbil, Iraq AD - Department of Biology, College of Science, University of Salahaddin, Erbil, Iraq Y1 - 2022 PY - 2022 VL - 7 IS - 26 SP - 34 EP - 38 KW - VitaminD KW - DNA Repair KW - MTH1 KW - MYH KW - Personalized Medicine DO - 10.22034/pmj.2022.698498 N2 - Vitamin D (Vit D) ,as an antioxidant contributes to a wide range of diseases including obesity, type 2 diabetes, multiple sclerosis, and certain cancers that oxidative stress plays a vital role in their development. Excessive oxidative stress can damage to DNA and nucleotide pool. Base excision repair and house-cleaning enzymes can protect genome so that any disruption in expression of these genes indicates enhanced susceptibility risk for diseases like cancer. The present study was conducted aimed at evaluating the effect of Vit D on the expression of MYH and MTH1 as DNA repair genes, as well as effect of ViD treatment in Human Umbilical Vein Endothelial Cells (HUVEC) cell line. To do this, bioinformatics tools were used to predict the interaction of MTH1 and MYH with VDR as a specific transcription factor (TF) for  Vit D. The cell line was treated with VitD. Next, viability was evaluated using MTT assay. The mRNA expression of MTH1 and MYH was assessed using real-time PCR at 48h post-treatment with Vit D.  Results of the study revealed that Vit D could regulate MTH1 and MYH  transcript expression directly through its specific TF; VDR. In response to VitD treatment a different alteration was observed in DNA repair, and non-canonical nucleotide repair genes. Findings of this study showed a new regulation of DNA repair genes in Vit D signaling pathway, and it may be a new perspective for the therapeutic effect of Vit D on related diseases. Variation in interested genes may affect the vitD signaling and personalized medicine should be considered.   UR - https://www.pmjournal.ir/article_698498.html L1 - https://www.pmjournal.ir/article_698498_6bacec652009da8c25b1218d55034f87.pdf ER -