AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201Investigating the relationship between gene CYP1B1 C4326G polymorphism and the risk of cervical cancer1324004210.22034/pmj.2020.240042ENFadhil SaidJarallah German Specialized ClinicMohammad Al-TohamiJarallah German Specialized ClinicVahidreza EsfahaniPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20201221 <br /><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi; color: black; background: white;">Cervical cancer is a common and deadly cancer among women around the world. </span><span style="mso-bookmark: _Hlk59459109;"><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi;">One of the genes associated with many cancers is the CYP1B1gene.</span></span><span style="mso-bookmark: _Hlk59459109;"><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi;">Some studies have shown that polymorphisms in the CYP1B1 gene can induce hyper activation of proteins and increase the incidence of several cancers. In the present study, we examined the association between the CYP1B1 C4326G polymorphism and the risk of cervical cancer.</span></span><span style="mso-bookmark: _Hlk59459109;"><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi;">60 newly diagnosed patients with cervical cancer and 60 cancers-free controls.</span></span><span style="mso-bookmark: _Hlk59459109;"><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi;">DNA was extracted by Salting-out method, Restriction fragment length polymorphism PCR was employed to determine the genotype of the CYP1B1 C4326G polymorphisms. the frequencies of the three genotypes (CC, CG, and GG) of CYP1B1 C4326G in cervical cancer cases and controls were 68.0, 25.4, 6.6%and 73.1, 21.4, and 5.5 %, respectively, and there was a significant difference between the two groups (χ2=7.41, P=0.02). <span style="mso-spacerun: yes;"> </span></span></span><br /> <br /><br /><br /><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-fareast-font-family: SimSun; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi; color: black; background: white; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: FA;">Cervical cancer,</span><span style="font-size: 12.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-ascii-theme-font: major-bidi; mso-fareast-font-family: SimSun; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: major-bidi; mso-bidi-theme-font: major-bidi; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: FA;">CYP1B1, RFLP-PCR, Human papilloma virus </span>https://www.pmjournal.ir/article_240042_d75e7c1ce81051c5486f0cd4813a51e1.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201The Association between Prostate Cancer and CXCL9 Gene Expression4624004310.22034/pmj.2020.240043ENAli MehdiZadehJarallah German Specialized ClinicMuhmmad Noor KazkazKuwait German Urology Unit(KGUU)Journal Article20201221Chemokine CXCL9 is a member of the CXC family and has an important role in the chemotaxis of immune cells. In this study, changes in the expression of gene CXCL9 in prostate cancer and adjacent healthy tissue was investigated. The prostate cancer tissues and the corresponding adjacent tissues used in this study were collected from 30 patients. qRT-PCR was performed for evaluated changes in the expression of gene CXCL9 in prostate cancer and adjacent healthy tissue. The mRNA levels of CXCL9 in prostate cancer samples was greater than normal samples (P=0.04), The results suggested that the mRNA expression levels of CXCL9 were positively associated with prostate cancer.https://www.pmjournal.ir/article_240043_e93908c8bb07ef8e86fc4bc211ed0d86.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201investigation the correlation between FGL1 expression and prognosis in gastric cancer patients7924004410.22034/pmj.2020.240044ENMahnaz SaremiReference health laboratory, Ministry of health and medical educationLeila MoezziDepartment of Cellular and Molecular, Faculty of Life Sciences, North Tehran Branch, Islamic Azad University, Faculty of Biological Sciences, Tehran, IranJournal Article20201221Gastric cancer is the fourth most common cancer worldwide and it ranks second among cancer deaths. Several studies have shown that FGL2 contributes to the pathogenesis of a number of infectious diseases, However, little is known about its biological functions in cancer development and metastasis. In this study, we investigated the correlation between FGL1 expression and prognosis in GC patients. gastric cancer tissue and adjacent healthy pecimens (n=20) were obtained from patients aged between 30 and 50 years who were diagnosed with in gastric cancer, Total RNA was extracted, Reverse transcription and qPCR was performed and Relative expression level was calculated using the 2-∆∆Cq method. We found that the expression of FGL1 in gastric cancer tissues were obviously higher than that in the adjacent tissues at mRNA levels (p <0.003).https://www.pmjournal.ir/article_240044_2ab8ee262728124b4cb8b0b9c762b2ef.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201MicroRNA 210 as a Biomarker in Breast Cancer101224004510.22034/pmj.2020.240045ENMohamad AlJarallahJarallah German Specialized ClinicNafise PoorhasanDepartment of Cellular and Molecular, Faculty of Life Sciences, North Tehran Branch, Islamic Azad University, Faculty of Biological Sciences, Tehran, IranJournal Article20201221The increasing literature exploring the role of circulating miR-210 has clarified its potential as a promising biomarker for early detection, diagnosis and prognosis. Measuring circulating miR-210 levels could be a non-invasive method for early cancer detection. Even if, to date, most studies appear to be preliminary it seems that miR-210 will be a good target for drug development. In this study, we had detected the difference of serum miR-210 expression between health and breast cancer patients. total of 15 breast cancer patients were selected for the breast cancer (BC) group and 15 healthy people. The results showed The expression level of miR-210 in the serum of breast cancer patients was significantly higher compared with that in healthy individuals. Patients with breast cancer present increased levels of circulating miR-210; thus, circulating miR-210 may be a potential biomarker of tumor presence and therapeutic response in breast cancerhttps://www.pmjournal.ir/article_240045_d236c9abe62d2007cbe9d0b2c0b00954.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201Evaluation of CENPM gene expression in cervical cancer131524004610.22034/pmj.2020.240046ENRaziye AmuzgarKermanshah University of Medical Sciences, School of the Medicine, Kermanshah, IranNajme ShojaeiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20201221Cervical cancer is the most widely screened cancer in the world both in high- and middle-income countries. HPV has been implicated in 99.7% of cervical squamous cell cancer cases worldwide. Viral E6 and E7 genes expression leads to alterations of the cellular genome integrity, including structural and numerical chromosomal instability resulting in chromosomal mis-segregation and aneuploidy. Centromere protein M (CENPM) is an essential centromere component for chromosome separation. In this study, we evaluated the expression of this gene at mRNA level by qRT-PCR method on 20 cancer samples who previously had feather papillomavirus infection, The results show a significant increase in the expression of this gene in cancer samples.https://www.pmjournal.ir/article_240046_1d5706bb1803fd1cca35f1cbed586a71.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201Titanium dioxide nanoparticles can induce apoptosis in cancer cells161824004710.22034/pmj.2020.240047ENHamed NaghoosiResearch Center for Cancer Epidemiology and Screening, Aja University of Medical Sciences, Tehran, Iran and Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, IranMohammad Ali SaremiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20201221Nanotechnology involves the creation and manipulation of materials at nanoscale to create products that exhibit novel properties. Nano-TiO2, as one of the top five nanoparticles in annual production, is widely applied in industries ranging from healthcare to drug delivery. The properties of nanoTiO2 have raised concerns regarding toxicity. Annexin V-FITC/PI double staining and a flow cytometer were used to detect apoptosis. A significant increase in apoptosis was observed at concentrations of TiO2-NP of 50-100 <em>µ</em>g/ml, varying between 25-40% apoptosis by 24 h. To further investigate the aptotic and toxic effects of these substances, it is recommended that this study be performed in the in vivo phase.https://www.pmjournal.ir/article_240047_8fc6127b281c9e6e5b2471714bd8bfb3.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851920201201Association of the KCNJ11 rs5219 E23K polymorphism with Type 2 Diabetes192124004810.22034/pmj.2020.240048ENMassoud HoushmandNational Institute of Genetic Engineering and Biotechnology NIGEB • Department of Medical BiotechnologyMohammed NajeebDeptofDiabetes& Internal Medicine, JarallahGerman Specialized Clinics, KuwaitJournal Article20201221Diabetes mellitus (DM) is a major public health issue in worldwide. Type 2 diabetes does not have a clear pattern of inheritance, although many affected individuals have at least one close family member, such as a parent or sibling, with the disease. The KCNJ11 gene is a member of the potassium channel gene family. polymorphisms in KCNJ11 result in neonatal diabetes and congenital hyper-insulinaemia, wherein the E23K (rs5219) polymorphism is linked with diabetes susceptibility where the K allele plays an important role in insulin secretion. In this study, we evaluate the frequency of these polymorphisms in a Kurdish population of 85 with type 2 diabetes. E23K polymorphism of KCNJ11 gene was genotyped by PCR-RFLP method. heterozygous carriers for AG are more in non‑diabetic patients (P = 0.034).https://www.pmjournal.ir/article_240048_c2cf1778ec4db1c0aa503c559c9fef72.pdf