AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Role of personalized microRNA-124 Expression in Ovarian Cancer153901410.21859/pmj04011ENBabak OtoukeshClinical Fellowship, Department of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, GermanyPeyman KaghazianDepartment of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, GermanyAmirjouya TalaeiDepartment of Genetics, Faculty of Life Science, Azad University of Tehran Medical Sciences Branch, Tehran, IranBahram BoddouhiBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranBahareh HeshmatDepartment of Radiology, Zahedan University of Medical Science, Zahedan, IranJournal Article20200415Introduction: MicroRNA-124 (miR-124) is moderated in some human malignancies and is associated with tumor advancement. But, its expression and clinical importance in ovarian carcinoma is still unclear. Thus, the goal of this study was to feature the clinical importance of personalized miR-124 expression in ovarian carcinoma. Methods: 94 women ovarian cancer tissues and 26 normal ovarian tissues were accumulated from patients. We used Real-time PCR to quantify the expression of personalized miR-124 in clinical ovarian carcinoma specimen and normal tissues. Moreover, we measured the miR-124 relationship with clinicopathologic characteristics and the ovarian carcinoma survival. Results: The lesser expression of miR-124 in tumor tissues can be found in compared with normal tissue using PCR method (P < 0.05). Our data exhibited that there is a notable association among low expression of miR-12 and clinical staging of ovarian carcinoma (P = 0.023). Nevertheless, miR-124 expression was not notably associated with age (P = 0. 671), differentiation status (P = 0.512), lymph node metastasis (P = 0.415) and histological subtypes (0.547). Kaplan-Meier survival analysis and log-rank test were applied in present study. These tests showed the less expression on patients had markedly short-term survival time in comparison with high expression group (P = 0.022). Multivariate Cox proportional hazards model analysis revealed that less expression of miR-124 and clinical staging were contribute to short-term survival in patients with ovarian carcinoma. The HR of the low miR-124 expression group was calculated to be 2.532 (95% CI: 1.572-9.237, P = 0.021), (clinical staging HR: 2.532; 95% CI: 1.321-9.241, P = 0.032).<br />Conclusions: These findings suggested that personalized miR-124 could be considered as an independent prognostic factor for ovarian carcinoma patients. Our findings suggested that low expression of personalized miR-124 has prognostic worthiness in ovarian.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Is History of Coronary Artery Bypass Graft Surgery a Strong Determinant of Inferiority of Organ in Cadaveric Liver Donation? Emerging Role of personalized Medicine673901510.21859/pmj04012ENMeysam MojtabaeeOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences
(SBMU), Tehran, IranSaman NikeghbalianShiraz Transplantation Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
(SBMU), Tehran, IranShagin ShahryariOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences
(SBMU), Tehran, IranSiavash GholamiOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences
(SBMU), Tehran, IranFarahnaz Sadegh BeigeeOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences
(SBMU), Tehran, IranJournal Article20200415Nowadays, there has been a considerable advance of personalized medicine on a<br />scientific basis and clinical demands in cardiovascular diseases. This study investigated<br />a history of coronary artery bypass graft surgery a strong determinant of inferiority of<br />organ in cadaveric liver donation. In this study, fate of 14 potential deceased liver<br />donors with a history of coronary artery bypass graft surgery has been investigated.<br />This report shows that careful gross and microscopic investigation of the liver is the<br />key to extract suitable life savior livers from donors with advanced age.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Personalized Medicine Journal in 2019: Reflecting on the Development of the Upcoming Role of Personalized Medicine in the Therapies893901610.21859/pmj04013ENMassoud HoushmandNational Institute of Genetic Engineering and BiotechnologySeyed Hassan SaadatBehavioral Sciences Research Center, lifestyle Institute, Baqiyatallah University of Medical Sciences, Tehran, IranJournal Article20200415In recent decades, there has been an increase in the incidence of cancer in the affected communities. However, the growth of therapeutic strategies has been very slow. Conventional diagnosis and subsequent treatment in medical centers originated from pathological based examinations, symptoms, and medications.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Clinical Significance and Seroprevalence of L. monocytogenesin in Pregnant Women with Spontaneous Abortion: Personalized Medicine to Improve Outcome (Diagnosis and Monitoring)10133901710.21859/pmj04014ENMahsa MozaffariDepartment of Pediatrics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IranMohamad MozafariGeneral Practitioner (GP), Tabriz University of Medical Sciences, Tabriz, IranMahdi MohebbiGeneral Practitioner (GP), Shahid Beheshti University of Medical Sciences, Tehran, IranJournal Article20200415Introduction: Recent scientific promotion reveals that medicine is currently at a transition stage from programmatic to personalized handling on in infectious diseases. Methods: Herrien, this research was performed to evaluation seropositivity for L. monocytogenes based on personalized medicine among the women with and without annals of miscarriage by indirect immunofluorescence test (IIFA). Moreover, the relationship of seropositivity with demographic factors was also investigated. Results: Our outcome showed that 26.92% (35 cases) of women with an involuntary miscarriage were seropositive for L. monocytogenes, while 11% (11 cases) of healthy pregnant women were found positive for L. monocytogenes (P = 0.001). The most case of listeria has been found in age group of 30-35 years old, but no notable difference was found among different groups (P = 0.245). Moreover, there was a considerable association between listeria occurrence and history of abortion when compared with healthy pregnant women (P = 0.001). In addition, early abortion and the number of pregnancy were significantly linked to Listeria- seropositive in patients with spontaneous abortion (P = 0.041; P = 0.034). Conclusions: Further researches are required to appraise the clinical importance of L. monocytogenesin in pregnant women with symptomatic and asymptomatic infection related to personalized medicine.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Evaluation of Personalized Medicine for Patients with Brain and Spinal Cord Injury using the Nigella sativa L. Seed: An Updated Comprehensive and Critical Review14183901810.21859/pmj04015ENBabak OtoukeshClinical Fellowship, Department of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, GermanyBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranPeyman KaghazianDepartment of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, GermanyBahram BoddouhiBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, IranBahareh HeshmatDepartment of Radiology , Zahedan University of Medical Science , Zahedan, IranMaria KaghazianDepartment of Biology, Jundishapur University of Medical Sciences, Ahvaz, IranJournal Article20200415Introduction: Nigella sativa (NS) is a widely used medicinal plant and appears to have a general health protective effect. As has been reported previously, pharmacological actions of NS have been explored including antidiabetic, anticancer, immunomodilative, analgesic, anti-inflammatory, spasmolytic, bronchodilator, hepatoprotective, renal protective, antioxidant properties, gastroprotective, antihistaminic, antibacterial, neuroprotective and antioxidative effects and etc. Methods: The present review aimed to give a personalized care for patients using detailed survey of the literature on and neuroprotective activities of the plant. Pubmed, Science Direct, Google scholar and Springer databases were searched from 1983 till January 2015. Key words were included: N. sativa, black seed, neuropathy, neuroprotective, brain and spinal injury, thymoquinone and posttrauma. Searching was limited to articles with English language. Review articles, case reports, abstract in symposium and congress, studies on N. sativa mixed with other plants were excluded. This study thus launches a huge resource for understanding the role of NS in in brain and spinal cord tissue damage after trauma with broad relevance for personalized medicine. Results: Results indicated that based on personalized medicine many of the herbal attributes of the herbal are due to the thymoquinone presence as its bioactive component, and therapy with NS notably decreased post-traumatic degenerative neurons and distorted nerve cells were not primarily treated in NS rats. In conclusion, NS can improve neuropathic status and neurological dysfunctions in the brain and spinal injury models. However, more clinical trials are necessary to clarify beneficial effects of NS its effective type and dosage for neuropathies management and its complications. Conclusions: Finally, NS treatment might be effective in detrimental the cerebral and spinal cord after trauma as personalized care for patients, and therefore shows potential for clinical implications.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Investigation of Toxoplasma gondii in Pregnant Women: A Strategy for Personalized Medicine19223901910.21859/pmj04016ENMahsa MozaffariDepartment of Pediatrics, Iran University of Medical Sciences, Tehran, IranMohamad MozafariGeneral practitioner (GP), Tabriz University of Medical Sciences, Tabriz, IranMorvarid OtoukeshGeneral Practitioner (GP), Shahid Beheshti University of Medical Sciences, Tehran, IranMohammad GhaemiDepartment of Anesthesiology, Iran University of Medical Science, Tehran, IranMahdi MohebbiGeneral Practitioner (GP), Shahid Beheshti University of Medical Sciences, Tehran, IranJournal Article20200415Introduction: In this study, we evaluated the Seroepidemiology of T. gondii among470 pregnant women as well as association of infection with socio-demographic factors and risk factors such as age, and education was studied, which makes it a potential therapeutic option for personalized medicine. Methods: This cross sectional study was conducted among 470 pregnant women who presented to health centers from February 2013 to January 2014. Serum samples were prepared via a commercial ELISA kit (Euroimmun, Germany) for the attendance of IgG and IgM toxoplasma antibodies and the avidity of the IgG antibody based on the manufacturer’s protocol. Results: We found 34.4% of Toxoplasma IgG. Among 470 pregnant women, 166 cases positive for IgG antibodies toxoplasma were detected, showing a serum incidence of 35.31% (95% confidence interval 27.8 to 37.06%),and thirty eight (22.89%) out of 166 IgG-positive women revealed specific IgM antibodies. Conclusions: Our data showed that the prevalence of T. gondii infection is not related to age, gestational age, number of pregnancies, history of abortion, contact with soil, life in rural areas and education related to infection. as well as these findings may be of major interest for the select of the first-line anti-infection drug, and the urgent require for developing personalized medicine.AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553841320190601Impact of diseased Donors’ Serum Creatinine Level on Outcome of transplanted Kidneys: The Use of Personalized Medicine for Patient Selection23253902010.21859/pmj04017ENMeysam MojtabaeeOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences (SBMU), Tehran, IranFariba GhorbaniOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences (SBMU), Tehran, IranFarahnaz Sadegh BeygiOrgan Procurement Unit (OPU), Lung Transplantation Research Center (LTRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences (SBMU), Tehran, IranJournal Article20200415Introduction: One of the possibilities is the utilize of personalized medicine, a method based on quantifiable and theoretical agents that assign the worldwide immunological hazard of refusal for each patient. Kidney transplantation is the best treatment for end-stage renal disease patients. Although the shortage of kidneys for transplantation has been addressed by expanding the criteria, transplantation teams in Iran don’t have great tendency to implant marginal organs from brain death donors specially the ones with high creatinine before harvesting. The aim of this study was to evaluate the impact of brain dead donor serum creatinine (Cr) level on outcome of transplanted kidneys. Methods: In this retrospective study, the records of 46 brain-dead donors of Masih Daneshvari organ procurement unit of Tehran, Iran were assessed. The medical file of donated 58 recipients was available. Donor demographic data (age, sex), brain death cause and the Cr level after transferring to OPU ICU were recorded. The Cr level was categorized based on ≥1.4 mg/dl with origin of prerenal azotemia or lower. The urine output of transplanted patients in the first 24 hours post-transplant, serum Cr level at discharge from hospital and dialysis during first month after transplantation were compared between two groups. Results: The mean age of donors was 37yr and 25 (54%) were male. The cause of brain death in 16 (35%) was trauma. The donors’ creatinine level at the admission to the ICU was 1.4mg/dl or higher in 20 (35%). The first 24 hours urine output did not significantly differ between donors’ creatinine level lower than 1.4mg/dl or higher (5500 ± 2678ml and 4068 ± 3399ml, respectively, P=0.083). Similarly the recipient discharge serum Cr level did not significantly differ between two groups (1.69 ± 0.97 and 1.74 ± 1.34 mg/dl, respectively, P=0.887). Overall 8 recipients (14%) needed dialysis during first month after transplantation and there was no significant difference between groups (11% and 20%, respectively, P = 0.320).<br />Conclusions: Findings of this preliminary study showed that the outcome of transplanted kidneys from brain-dead donors with Cr level >=1.4mg/dl is similar to other patients. So, higher creatinine level is not a good excuse for rejecting the kidneys for transplantation. Because of various confounding factors in the assessment of transplanted kidney outcome, the future studies with larger sample size and longer follow-up period is recommended.