Investigating the relationship between gene CYP1B1 C4326G polymorphism and the risk of cervical cancer

Document Type : Original Article

Authors

1 Jarallah German Specialized Clinic

2 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

10.22034/pmj.2020.240042

Abstract

 
Cervical cancer is a common and deadly cancer among women around the world. One of the genes associated with many cancers is the CYP1B1gene.Some studies have shown that polymorphisms in the CYP1B1 gene can induce hyper activation of proteins and increase the incidence of several cancers. In the present study, we examined the association between the CYP1B1 C4326G polymorphism and the risk of cervical cancer.60 newly diagnosed patients with cervical cancer and 60 cancers-free controls.DNA was extracted by Salting-out method, Restriction fragment length polymorphism PCR was employed to determine the genotype of the CYP1B1 C4326G polymorphisms. the frequencies of the three genotypes (CC, CG, and GG) of CYP1B1 C4326G in cervical cancer cases and controls were 68.0, 25.4, 6.6%and 73.1, 21.4, and 5.5 %, respectively, and there was a significant difference between the two groups (χ2=7.41, P=0.02).  
 


Cervical cancer,CYP1B1, RFLP-PCR, Human papilloma virus

Keywords