Introduction: In this study, we evaluated the Seroepidemiology of T. gondii among470 pregnant women as well as association of infection with socio-demographic factors and risk factors such as age, and education was studied, which makes it a potential therapeutic option for personalized medicine. Methods: This cross sectional study was conducted among 470 pregnant women who presented to health centers from February 2013 to January 2014. Serum samples were prepared via a commercial ELISA kit (Euroimmun, Germany) for the attendance of IgG and IgM toxoplasma antibodies and the avidity of the IgG antibody based on the manufacturer’s protocol. Results: We found 34.4% of Toxoplasma IgG. Among 470 pregnant women, 166 cases positive for IgG antibodies toxoplasma were detected, showing a serum incidence of 35.31% (95% confidence interval 27.8 to 37.06%),and thirty eight (22.89%) out of 166 IgG-positive women revealed specific IgM antibodies. Conclusions: Our data showed that the prevalence of T. gondii infection is not related to age, gestational age, number of pregnancies, history of abortion, contact with soil, life in rural areas and education related to infection. as well as these findings may be of major interest for the select of the first-line anti-infection drug, and the urgent require for developing personalized medicine.