Despite the increasing number of diabetic patients, self-care plays an important role in the prevention and detection of various complications such as neurological disorders. The aim of this experiment was to investigate the main factors affecting diabetes type 1 and self-care. For this purpose, six volunteer subjects with diabetes type 1 were included. Their glucose levels together with the carbohydrate intake and other factors were recorded four times a day for 30 days. In order to perform statistical analysis, the one-way variance analysis, Pearson correlation coefficient, time series analysis, and the combined time series (panel) analysis were applied. The findings of this study demonstrated that the alteration in blood glucose levels was strongly influenced by carbohydrate intake, physical activity, stress level, amount of sleep, and insulin; while slightly influenced by pills and supplements use, hypoglycemia, insulin sensitivity, alcohol and cigarette use, and comorbidity. In four patients, the self-care score was normal, one patient exhibited high level and the reaming showed low level. For each patient, the error rate was as follows: 6.451, 6.095, 8.819, 7.368, 6.05, 5.856. Regarding to the loss of HbA1c rate after our study, people are advised to control their blood glucose levels based on individual preferences, conditions, lifestyle, and physiology for preventing severe diabetes type 1 conditions and extra cost.