Document Type : Original Article
Authors
1 Department of Biology/ Salahaddin University-Erbil Verified email at su.edu.krd
2 Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran
3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran
Abstract
Cervical cancer is the second most common cancer and an important cause of death in women worldwide. Objective biomarkers are needed to improve specificity for cervical cancer screening. The p16 gene is implicated in the cell cycle control, playing an important role as a tumor suppressor gene. In this study, the methylation of the P16 gene promoter was evaluated in people with cervical cancer and people with the papilloma virus. The study population included nine women with cervical cancer whose malignancy had been confirmed by a pathologist and ten patients with high-risk types of HPV virus. Methylation status was evaluated by MS-PCR. Cervical cancer patients showed a significantly higher methylation frequency for the p16 gene as compared to the control and the HPV group (p=0.001).
Keywords
2. Pisani P, Parkin DM, Bray F, Ferlay J. Estimates of the
worldwide mortality from 25 cancers in 1990. Int J Cancer 1999;
83: 18-29.
3. Walboomers JM, Jacobs MV, Manos MM, Bosch FX, Kummer
JA, Shah KV, et al. Human papillomavirus is a necessary cause of
invasive cervical cancer worldwide. J Pathol 1999; 189: 12-9.
4. Schiffman MH, Castle P. Epidemiologic studies of a necessary
causal risk factor: human papillomavirus infection and cervical
neoplasia. J Natl Cancer Inst 2003; 95: E2.
5. Bosch FX, Manos MM, Munoz N, Sherman M, Jansen AM,
Peto J, et al. Prevalence of human papillomavirus in cervical cancer:
a worldwide perspective. International biological study on cervical
cancer (IBSCC) study group. J Natl Cancer Inst 1995; 87: 796-802.
6. Waggoner SE. Cervical cancer. Lancet 2003; 361: 2217-25 .7.Holowaty P, Miller AB, Rohan T, To T. Natural history of
dysplasia of the uterine cervix. J Natl Cancer Inst 1999; 91: 252-8.
8. Bird A. DNA methylation patterns and epigenetic memory.
Genes Dev 2002; 16: 6-21.
9. Russo VEA, Martienssen RA, Riggs AD, eds. Epigenetic
mechanisms of gene regulation. Plainview, N.Y.: Cold Spring
Harbor Laboratory Press, 1996.
10. Yoder JA, Walsh CP, Bestor TH. Cytosine methylation and the
ecology of intragenomic parasites. Trends Genet 1997; 13: 335-40.
11. Baylin SB, Herman JG, Graff JR, Vertino PM, Issa JP.
Alterations in DNA methylation: a fundamental aspect of
neoplasia. Adv Cancer Res 1998; 72: 141-96
)