Document Type : Original Article
Fish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University, Erbil, Iraq
Department of biology, Nourdanesh institute of higher education, Meymeh, Isfahan, Iran
Department of Public Management, Faculty of Management, University of Shahrekord, Iran
Personalized Medicine Research Center of AmitisGen, Tehran, Iran
The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method.