Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.
M. Mohammed, F., Pakzad, H., Esfahani, V. (2020). Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis. Personalized Medicine Journal, 5(17), 9-11. doi: 10.22034/pmj.2020.43453
MLA
Fawziah M. Mohammed; Hossein Pakzad; Vahid Reza Esfahani. "Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis". Personalized Medicine Journal, 5, 17, 2020, 9-11. doi: 10.22034/pmj.2020.43453
HARVARD
M. Mohammed, F., Pakzad, H., Esfahani, V. (2020). 'Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis', Personalized Medicine Journal, 5(17), pp. 9-11. doi: 10.22034/pmj.2020.43453
VANCOUVER
M. Mohammed, F., Pakzad, H., Esfahani, V. Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis. Personalized Medicine Journal, 2020; 5(17): 9-11. doi: 10.22034/pmj.2020.43453
)