Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis

Authors

1 Faculty of Allied Health Sciences KuwaitnMedical Laboratory Sciences

2 Department of biology, Tehran-east branch, Islamic azad university, Tehran, Iran

3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

Abstract

Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.

Keywords