Personalized and Precision Medicine Journal

Abstract Free radicals are naturally produced in the body and are inhibited by the body’s antioxidants. The excessive production of free radicals and the inability of the body to remove them lead to oxidative stress in the body, which can lead to many diseases, including cancer. Nanoparticles are compounds that have been given much attention to cancer prevention and treatment, due to their specific biological characteristics and their small size. This study aimed to evaluate the cytotoxic and antioxidant potential of serum oxide nanoparticles synthesized using aqueous extract of Hyssopus officinalis.
To perform the MTT assay, first, the MDA-MB231 cancerous cells were cultured, seeded and then treated for 24, 48 and 72 hours. Subsequently, MTT was performed and finally, absorption at 517 nm was recorded. The antioxidant potential of the CeO-NPs was evaluated by estimating the amount of ABTS and DPPH free radicals inhibiting in different concentrations of nanoparticles.
The results showed that the CeO-NPs were able to inhibit the ABTS and DPPH free radicals with a mean concentration (IC50) of about 62 and 31.2 μg / ml. Also, the CeO-NPs inhibited cancer cells with IC50 of about 400 µg/ml, 48 hours after exposure. According to the antioxidant results obtained from this paper, it is suggested that by performing further experiments, this nanoparticle can be used as an antioxidant supplement.

Abstract Background: Leishmania Species produced diseases include clinical problems from cutaneous self-limiting to severe non-healing forms such as visceral leishmaniasis (VL). As an obligatory intracellular parasite these pathogens proliferate and survive inside macrophages in animals and human; while these cells as a major host immune cell destroy majority of disease producing agents. Because macrophages act as first line of innate immunity, produce several molecules when activated. Proinflammatory and inflammatory cytokines are produced by these cells through their activation, act as main coordinators of the immune system against pathogens and other harmful disease producing factors against the body. Through such a mechanism the immune response resolves the problem.  To play such a critical role many cells as monocytes, macrophages, DCs and others involved in T cell regulation to establish proper innate and adaptive immunity responses. Proinflammatory and inflammatory cytokines are produced in a network acting through many signal pathways.
Methods: In this descriptive designed study, quality-controlled cDNA samples sequenced (RNA-seq) and mapped against a standard human genome version. Results: Based on the results of this study, proinflammatory and inflammatory gene expressions were significantly upregulated.
Conclusion: Upregulations of proinflammatory and inflammatory gene expressions early infection time might be indication for an early  innate immunity response.