Personalized and Precision Medicine Journal

Abstract Chancroid is an STI characterized by the Gram-negative bacteria Haemophilus ducreyi. Controlling chancroid is challenging, and the primary treatment accessible is antimicrobial therapy. However, drug resistance has been seen in places where the disease is common. Due to recent global outbreaks of sexually transmitted infections (STIs), it is crucial to continue researchi ng innovative treatment options and prevention measures. We used reverse vaccinology and subtraction genomic methods to determine potential vaccination and therapeutic targets against H. ducreyi in silico. We found 56 Secreted proteins, with 159 membrane molecules and 515 cytoplasmic proteins. We assessed their need, operation, and ability to cause disease. We identified 6 potential vaccination targets and three pharmacological targets inside pathogenicity islands. The discovered targets may be utilized in future initiatives to manage chancroid globally.