Personalized and Precision Medicine Journal

Abstract Obesity and type 2 diabetes are two complex disorders with strong genetic backgrounds, and both disorders cause other diseases and increase mortality. FTO is a protein-coding gene located on the long arm of chromosome 16 and linked to type 2 diabetes and obesity. The current study investigated the relationship between the RS16953002 polymorphism gene and type 2 diabetes and obesity in a population from Tehran. The study population included 150 people with type 2 diabetes and 150 healthy individuals. Genotyping was performed by RFLP-PCR. The results indicated the presence of the GG allele in 81% of diabetic patients but in only64% of non-diabetic participants, which shows a statistically significant difference in this regard. Thus, the current study has shown the role of FTO gene polymorphisms in the pathogenesis of people with type 2 diabetes and obesity.

Abstract Nonalcoholic fatty liver disease (NAFLD) is a common cause of chronic liver disease. Non-alcoholic fatty liver disease is a spectrum of simple steatosis that is benign to non-alcoholic steatohepatitis (NASH) associated with fibrosis and inflammation. Genetic factors play an important role in this disease. The PNPLA3 gene (C > G) rs738409 polymorphism has been defined to be associated with increased liver fat content and liver damage in metabolic disorders. In this study, the association of this polymorphism with non-alcoholic fatty liver disease was investigated in the Mazandaran province population. Our study population consisted of 85 patients and 85 controls. Blood samples were collected from patients and DNA were then extracted by boiling method. Genotypes of this polymorphism were determined by PCR-RFLP method. There was a significant relationship between CG genotype and risk of NAFLD (P = 0.012).