Personalized and Precision Medicine Journal

Abstract The roles of miRNAs in autoimmunity are only beginning to be explored; they may be involved in regulating immune responses against self-tissues.miRNAs may contribute to disease progression and response to treatment in MS patients. Several studies on MS have analyzed the role or profile of miRNAs in different tissues including peripheral blood mononuclear cells (PBMCs).The current study evaluated mir-21 and mir-146a in CSF samples of patients with multiple sclerosis. Differential expression of the two miRNAs was detected in at least 80% of the CSF samples;however, additional functional studies and analyses of larger cohorts are needed to validate these results and to elucidate the real role of these miRNAs in the context of MS.
 

Abstract Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.

Abstract In many cancers, an increase in gene expression of DNMT3b has been reported. This enzyme inhibits the expression of many tumor suppressor genes through the methylation of the promoter sequence and plays a key role in the progression of cancer. In this study, the relationship between polymorphism rs406193 of this gene and the risk of prostate cancer in 60 samples was investigated. The results showed a significant association between genotype TT and the risk of prostate cancer (p=0.048). It is recommended that this study be repeated in larger populations and that the relationship between this polymorphism and gene expression be investigated.