Personalized and Precision Medicine Journal

Abstract Cervical cancer is the most widely screened cancer in the world both in high- and middle-income countries. HPV has been implicated in 99.7% of cervical squamous cell cancer cases worldwide. Viral E6 and E7 genes expression leads to alterations of the cellular genome integrity, including structural and numerical chromosomal instability resulting in chromosomal mis-segregation and aneuploidy. Centromere protein M (CENPM) is an essential centromere component for chromosome separation. In this study, we evaluated the expression of this gene at mRNA level by qRT-PCR method on 20 cancer samples who previously had feather papillomavirus infection, The results show a significant increase in the expression of this gene in cancer samples.

Abstract Vitamin C plays is a cofactor for enzymes involved in many processes and has effects that are important for cancer transformation, such as antioxidant defense, transcription, and epigenetic regulation of gene expression.Angiogenesis is a normal process required for normal tissue repair and growth. Pathological angiogenesis is characterized by the persistent proliferation of endothelial cells and formation of blood vessels.The current study evaluated the effect of ascorbic acid on angiogenesis by investigating the expression of genes related to angiogenesis after treatment with different doses of ascorbic acid. By changing the concentration and administration time of ascorbic acid, a positive effect on the growth and metastasis of cancer cells in the group injected with ascorbic acid prior to having cancer cells injected into the abdominal cavity .



 

Abstract The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method.

Abstract PAI-1 has become recognized as a central molecule linking pathogenesis and progression of thrombotic vascular events, including stroke. Clinical and experimental studies show that PAI-1 deficiencies cause accelerated fibrinolysis and bleeding, whereas elevated PAI-1 plasma levels are associated with vascular thrombosis. Raised PAI1 plasma levels are related to a 1-bp guanine deletion/insertion (4G/5G) polymorphism in the promoter of the PAI1 gene. The 4G allele is associated with higher plasma PAI1 transcription and activity. In the current study, the association of higher PAI-1 plasma levels and the prevalence of the 4G/5G polymorphism in the PAI-1 gene promoter region in young patients with stroke were explored. Significantly, higher PAI-1 levels were observed in patients when compared to controls (p =002). The 4G/5G polymorphisms were significantly associated with increased PAI-1 levels, with the variant homozygous 4G/4G corresponding to mean values in patients versus controls.

Abstract In many cancers, an increase in gene expression of DNMT3b has been reported. This enzyme inhibits the expression of many tumor suppressor genes through the methylation of the promoter sequence and plays a key role in the progression of cancer. In this study, the relationship between polymorphism rs406193 of this gene and the risk of prostate cancer in 60 samples was investigated. The results showed a significant association between genotype TT and the risk of prostate cancer (p=0.048). It is recommended that this study be repeated in larger populations and that the relationship between this polymorphism and gene expression be investigated.