Personalized and Precision Medicine Journal

Abstract Vitamin C plays is a cofactor for enzymes involved in many processes and has effects that are important for cancer transformation, such as antioxidant defense, transcription, and epigenetic regulation of gene expression.Angiogenesis is a normal process required for normal tissue repair and growth. Pathological angiogenesis is characterized by the persistent proliferation of endothelial cells and formation of blood vessels.The current study evaluated the effect of ascorbic acid on angiogenesis by investigating the expression of genes related to angiogenesis after treatment with different doses of ascorbic acid. By changing the concentration and administration time of ascorbic acid, a positive effect on the growth and metastasis of cancer cells in the group injected with ascorbic acid prior to having cancer cells injected into the abdominal cavity .



 

Abstract The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method.