Personalized and Precision Medicine Journal

Abstract Human Papillomavirus (HPV) is a highly prevalent virus responsible for several types of cancers, including cervical, throat, and anogenital cancers. Early detection and diagnosis are crucial for preventing the progression of HPV-related diseases. In this study, we introduce a new approach based on Förster Resonance Energy Transfer (FRET) method to identify viral DNA, was designed for the conserved region of the L1 gene sequence in high-risk genotypes 16, 18, 31 and 33. In order to create suitable temperature conditions for the attachment and also to identify the fluorescent signal, real time PCR device was used. The results of the specificity test showed 100% specificity and the limit of detection level of the method was reported to be 1000 copies/µl of the virus in the sample. The results of clinical sensitivity in the range of 86-96% between deferent genotype and the rate of false negative results was in the range of 14-22%. Based on this, it can be said that maybe the developed method cannot be proposed as a suitable alternative, but due to the response time and lower cost, it can be proposed as a quick screening method.

Abstract Hysterectomy, the surgical excision of the uterus, has historically been fundamental in the management of gynecologic malignancies, encompassing endometrial, cervical, and ovarian cancers. Traditionally, hysterectomy has been executed via open abdominal surgery, a method that, although efficacious, frequently leads to extended recovery periods, heightened risk of complications, and greater psychological and physical strain for patients. In recent decades, substantial breakthroughs in surgical techniques and technology have fundamentally transformed the approach to these treatments, resulting in enhanced clinical outcomes, diminished complications, and expedited recovery periods. This study examines recent advancements in hysterectomy techniques, particularly highlighting the rise of minimally invasive approaches, including laparoscopic, robotic-assisted, and vaginal hysterectomy. These methods, characterized by smaller incisions and improved vision, are linked to many benefits, such as less blood loss, abbreviated hospital stays, and expedited resumption of normal activities. Furthermore, we examine the technical innovations that have significantly enhanced surgical precision, including 3D imaging, intraoperative molecular imaging, and real-time navigation systems.
These innovations have not only enhanced the effectiveness of treatment but have also contributed to a better overall quality of life for patients by reducing postoperative pain, minimizing scarring, and offering quicker recovery. Moreover, the psychological and emotional strains associated with cancer surgery, such as anxiety and body image concerns, are mitigated by less invasive techniques, resulting in enhanced patient satisfaction. Through this comprehensive analysis, the review highlights the transformative role of these technological and surgical advancements in gynecologic cancer treatment. These advancements enhance the precision and results of hysterectomy, reducing the long-term physical and psychological difficulties typically linked to cancer procedures, ultimately leading to improved treatment experiences and increased survival rates.

Abstract TERT It has been shown that TERT is overexpressed in 90% of human cancers, and genetic alterations in the proximal promoter of TERT are significantly associated with a variety of different cancer types. In recent years, a new mechanism of TERT regulation through the non-coding driver mutations (C228T and C250T) in the TERT promoter has been reported in several cancer types. In the present study, we investigated the relationship between the Single Nucleotide Polymorphism (SNP) rs2853669 and cervical cancer.
The study included 80 individuals, including 50 patients with cervical cancer and 30 healthy controls. The samples from the participants underwent sequencing and genotyping using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method.
It was found that 16%, 24%, and 60% of the cervical cancer samples had the genotypes of AA, AG, and GG, respectively. In the control group, the frequencies were 13.33%, 50%, and 36.66% of the samples for the genotypes of AA, AG, and GG, respectively. 
According to our findings, there was a significant association between the recessive model GG vs. AA+AG and cervical cancer susceptibility.
 

Abstract  
Cervical cancer is a common and deadly cancer among women around the world. One of the genes associated with many cancers is the CYP1B1gene.Some studies have shown that polymorphisms in the CYP1B1 gene can induce hyper activation of proteins and increase the incidence of several cancers. In the present study, we examined the association between the CYP1B1 C4326G polymorphism and the risk of cervical cancer.60 newly diagnosed patients with cervical cancer and 60 cancers-free controls.DNA was extracted by Salting-out method, Restriction fragment length polymorphism PCR was employed to determine the genotype of the CYP1B1 C4326G polymorphisms. the frequencies of the three genotypes (CC, CG, and GG) of CYP1B1 C4326G in cervical cancer cases and controls were 68.0, 25.4, 6.6%and 73.1, 21.4, and 5.5 %, respectively, and there was a significant difference between the two groups (χ2=7.41, P=0.02).  
 


Cervical cancer,CYP1B1, RFLP-PCR, Human papilloma virus

Abstract Cervical cancer is the most widely screened cancer in the world both in high- and middle-income countries. HPV has been implicated in 99.7% of cervical squamous cell cancer cases worldwide. Viral E6 and E7 genes expression leads to alterations of the cellular genome integrity, including structural and numerical chromosomal instability resulting in chromosomal mis-segregation and aneuploidy. Centromere protein M (CENPM) is an essential centromere component for chromosome separation. In this study, we evaluated the expression of this gene at mRNA level by qRT-PCR method on 20 cancer samples who previously had feather papillomavirus infection, The results show a significant increase in the expression of this gene in cancer samples.

Abstract Cervical cancer is the second most common cancer and an important cause of death in women worldwide. Objective biomarkers are needed to improve specificity for cervical cancer screening. The p16 gene is implicated in the cell cycle control, playing an important role as a tumor suppressor gene. In this study, the methylation of the P16 gene promoter was evaluated in people with cervical cancer and people with the papilloma virus. The study population included nine women with cervical cancer whose malignancy had been confirmed by a pathologist and ten patients with high-risk types of HPV virus. Methylation status was evaluated by MS-PCR. Cervical cancer patients showed a significantly higher methylation frequency for the p16 gene as compared to the control and the HPV group (p=0.001).