Personalized and Precision Medicine Journal

Abstract  
Cervical cancer is a common and deadly cancer among women around the world. One of the genes associated with many cancers is the CYP1B1gene.Some studies have shown that polymorphisms in the CYP1B1 gene can induce hyper activation of proteins and increase the incidence of several cancers. In the present study, we examined the association between the CYP1B1 C4326G polymorphism and the risk of cervical cancer.60 newly diagnosed patients with cervical cancer and 60 cancers-free controls.DNA was extracted by Salting-out method, Restriction fragment length polymorphism PCR was employed to determine the genotype of the CYP1B1 C4326G polymorphisms. the frequencies of the three genotypes (CC, CG, and GG) of CYP1B1 C4326G in cervical cancer cases and controls were 68.0, 25.4, 6.6%and 73.1, 21.4, and 5.5 %, respectively, and there was a significant difference between the two groups (χ2=7.41, P=0.02).  
 


Cervical cancer,CYP1B1, RFLP-PCR, Human papilloma virus

Abstract Cervical cancer is the most widely screened cancer in the world both in high- and middle-income countries. HPV has been implicated in 99.7% of cervical squamous cell cancer cases worldwide. Viral E6 and E7 genes expression leads to alterations of the cellular genome integrity, including structural and numerical chromosomal instability resulting in chromosomal mis-segregation and aneuploidy. Centromere protein M (CENPM) is an essential centromere component for chromosome separation. In this study, we evaluated the expression of this gene at mRNA level by qRT-PCR method on 20 cancer samples who previously had feather papillomavirus infection, The results show a significant increase in the expression of this gene in cancer samples.