TY - JOUR ID - 249038 TI - Induction of Bax and Caspase-3 Genes Expression by Simultaneous Administration of Phycocyanin and Citrullus Colocynthis Extract in Human Colorectal Cell Line (HT-29) JO - Personalized Medicine Journal JA - PMJ LA - en SN - 2476-5538 AU - Darabi, Fatemeh AU - Hassanpour, Halimeh AU - Maghami, Parvaneh AU - Mohebbi, Sohameh AU - Arabzadeh, Somayeh AD - Department of Biology, Faculty of Basic science, Science and Research Branch, Islamic Azad university AD - Aerospace Research Institute, Ministry of Science Research and Technology, Tehran 14665-834, Iran AD - Department of Biology, Faculty of Basic science, Science and Research Branch, Islamic Azad AD - Department of Biology, Faculty of Basic science, Ale Taha institute of higher education. Y1 - 2021 PY - 2021 VL - 6 IS - 23 SP - 17 EP - 24 KW - Citrullus Colocynthis Extract (Abujahl Watermelon) KW - Phycocyanin KW - Bax KW - Caspase 3 KW - Colon cancer DO - 10.22034/pmj.2021.249038 N2 - Introduction: In this study the effect of C. colocynthis extract and phycocyanin on the growth of colon cancer cells, and the expression of Caspase 3 and Bax were investigated.Materials & Methods: colon cancer cells (HT29) were treated with different concentrations of C. colocynthis and phycocyanin extracts for 24, 48 & 72 hours. The percentage of cell survival was then measured by MTT assay. Expression of Caspase 3 and Bax genes was investigated by real-time PCR. At the end results were analyzed by SPSS software.Results: Significant differences were observed in the average percentage of dead cells with 2 to 30 g/μL concentration of extract, phycocyanin, extract + phycocyanin after 24, 48 and 72 hours (p<0.05). The combination of extract and phycocyanin as well as phycocyanin alone showed stronger inhibitory effects on growth of cancer cells compared to extract (p <0.05). The expression of Bax gene was significantly increased by treatment of combination of extract and phycocyanin (2.55-fold) and also C. colocynthis extract alone (1.67-fold) (p <0.01). In addition, the combination of extract and phycocyanin and Abujahl watermelon extract significantly increased the expression of caspase 3 gene (2.15 and 1.75), respectively (p <0.01).Conclusion: The anticancer effect of Citrullus Colocynthis extract, as well as phycocyanin can be applied by increase the expression of Bax and caspase 3 genes and as a result, apoptosis induction in cancer cells. UR - https://www.pmjournal.ir/article_249038.html L1 - https://www.pmjournal.ir/article_249038_87f7b9feeab4298106a2dd312a41a1b3.pdf ER -