AmitisGen TECH Dev Group Personalized and Precision Medicine Journal 3115-7874 5 17 2020 06 01 Comparison of different methods of DNA extraction from paraffin-embedded tissues 5 8 43452 10.22034/pmj.2020.43452 EN Ramadhan Ibrahim Fish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University, Erbil, Iraq Saeed Megdadi Department of biology, Nourdanesh institute of higher education, Meymeh, Isfahan, Iran Sareh Bakhshandeh Bavarsad Department of Public Management, Faculty of Management, University of Shahrekord, Iran Najme Shojaei Personalized Medicine Research Center of AmitisGen, Tehran, Iran Journal Article 2020 07 26 The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method. https://www.pmjournal.ir/article_43452_5d3f73c8fb54cf51fc0b674c3e2841fc.pdf