AmitisGen TECH Dev Group Personalized and Precision Medicine Journal 3115-7874 8 30 2023 09 28 Adjustment of a Fibrosis Marker, Pro-Inflammatory Cytokines, and IgE in Asthmatic Animals 32 40 707859 10.22034/pmj.2023.2009908.1010 EN Fereshteh Dalouchi Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran. Zeynab Sharifi Aghdam Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran Raza Falak Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Morteza Bakhshesh Khomein University of Medical Sciences, Khomein, Iran Maryam Hajidazeh Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Maryam Naseroleslami Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, &lrm;Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Mahdieh Mehrab Mohseni Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Yaser Azizi Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran. Nahid Aboutaleb Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran. 0000-0002-7514-5939 Journal Article 2023 05 07 <strong>Background</strong>: A lot of patients are suffering from asthma. For decreasing the asthma symptoms, we studied the effects of conditioned medium (CM) of human amniotic membrane mesenchymal stem cells (hAM-MSCs) as a source of anti-inflammatory cytokines on splenocyte and lung tissue of asthmatic Balb/c mice.<br><strong>Methods:</strong> Forty mice were categorized into four groups; ovalbumin (OVA)-induced asthma, CM-treated asthma, DMEM (Dulbecco's Modified Eagle Medium)-treated asthma, and saline control. Each group received related treatment. The lung alpha-smooth muscle actin (α-SMA)  and splenocyte inflammatory cytokines and IgE were examined through Western blot analysis.<br><strong>Results:</strong> Western blot showed α-SMA overexpression in the OVA and DMEM groups compared with the saline group. CM therapy could significantly reverse it compared with OVA and OVA+DMEM categories by elevating IL-10 and IFN-γ and reducing IL-4, IgE, and TGF-β .<br><strong>Conclusion:</strong> CM treatment could improve asthma symptoms by adjusting α-SMA in lung tissue and pro-inflammatory cytokines and IgE in splenocytes. https://www.pmjournal.ir/article_707859_dd482b494ff0641c71e1e709449f29e7.pdf