AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320Investigation of PTEN Promoter Methylation and Its Effect on Non-Small Cell Lung Carcinoma1324387210.22034/pmj.2021.243872ENMaryam HassanlouFaculty of Biology, Women's university of semnan (Farzanegan), Semnan, IranSohameh MohebbiDepartment of Biotechnology, Faculty of Basic Science, Ale-Taha Institute of Higher Education, Tehran, IranJournal Article20201220<em>PTEN</em> is a tumor suppressor gene with important roles in apoptosis. This gene is located on chromosome 10q23 and is one of the most frequently inactivated genes in cancers. Severe underexpression of <em>PTEN</em> has been reported in several types of cancer, including endometrial, prostate, breast, and brain cancer. Also, this gene is epigenetically silenced through aberrant hypermethylation of CpG islands on its promoter. The present study investigated the promoter methylation and expression levels of <em>PTEN</em> in patients with Non-Small Cell Lung Carcinoma (NSCLC).<br />The study included 20 patients with NSCLC, whose bisulfite-treated DNA samples were investigated using the Methyl-Specific PCR (MSP) with primers specific for either methylated or non-methylated forms of <em>PTEN</em>. <em>PTEN</em> expression was also assessed using real-time PCR.<br /> 20 samples from NSCLC patients, 70% (n=14) showed <em>PTEN</em> underexpression, while 85% (n=17) had<em> PTEN</em> promoter methylation. Also, 12 of 17 (70%) samples with PTEN promoter methylation had concomitant <em>PTEN</em> underexpression.<br />According to our results, there was a significant correlation between the <em>PTEN</em> promoter methylation and NSCLC. <em>PTEN</em> underexpression and promoter methylation were also significantly correlated.https://www.pmjournal.ir/article_243872_884d30bb4401ddb0a13b9376a18ffe4a.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320Personalized Medicine in Bipolar Disorder4824387510.22034/pmj.2021.243875ENParham PooladgarSchool of Medicine, Shahid Beheshti University of Medical sciencesBahar Naghavi GargariBasic Sciences Department, Shahid Beheshti University of Medical SciencesJournal Article20201218Bipolar Disorder (BD) is a cognitive and behavioral disease with mood fluctuation . The 6th global problem is in adults. Disease susceptibility is affected through genetic factors, the epigenetic process marked the disease phenotype. On the other hand, the importance of DNA methylation in some neurobiological and cognitive activities such as brain development processes and activity includes psychiatric diseases like BD. Numerous long intergenic noncoding RNAs were found that regulate gene expression of several diseases and are involved in the brain and cognitive development as well as psychiatric disorders such as BD.<br />Despite advances in neuropsychological or biological markers discoveries which predict personalized treatment efficacy, the clinical history and exhibition are careful and predictable markers for patient categorizing and treatment management. The aim of individualized medicine is to find vulnerability or preservative factors through genetic change.<br />Genetic, epigenetic factors, imaging, psychopathology and biomarkers can affect new treatments. Various studies such as family, twin, and adoption studies , linkage analysis indicated the association of HPA axis genes with vulnerability to BD. Personalized medicine applications in psychiatry focus on descriptive psychopathology and phenomenology via precise analysis and attention to each patient’s impaired brain and mood processes.<br />The precision medicine studies concentrate on response to lithium, main treatment of BD , frequent mood diseases , antidepressant resistant prediction ,risk and outcome assessment. Precision medicine is a hopeful way to develop new treatments based on individual genetic features. Personalized medicine in psychiatric disorder is in the infancy phases, but promising approaches were developed for complex diseases treatment with human genome sequencing.https://www.pmjournal.ir/article_243875_7f693b57ae34680f9dabec49d6a7f713.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320Association of C677T Single Nucleotide Polymorphism of MTHFR with Susceptibility to Autism Spectrum Disorders91124387610.22034/pmj.2021.243876ENRafid A AbdulkareemDepartment of Genetic Engineering and Biotechnology Institute, Baghdad University, Baghdad, IraqAbbas AL-MashhadiMedical Microbiology Branch College of Medicine, Kufa University Al-Najaf, IraqJournal Article20201229In general, people with Autism Spectrum Disorders (ASD) have problems in social, emotional, and communication skills. Genome-Wide Association Studies (GWAS) have suggested a potential association of the C677T polymorphism of Methylenetetrahydrofolate Reductase (<em>MTHFR</em>) with autism spectrum disorders. The present study intended to investigate the relationship between this polymorphism of <em>MTHFR</em> and the severity of autism symptoms in two groups of children affected by autism and healthy children to elucidate its potential role as a risk factor for ASD.<br />study included 40 patients with autism and 40 healthy participants with matched age as control. The samples from the participants underwent ARMS-PCR for <em>MTHFR</em><em> </em>genotyping. <br />The CC genotype was reported in 50% (n=20) and 72.50% (n=29) of the children in the study and control groups, respectively, while the CT genotype was observed in 35% (n=14) of the study group and 17.50% (n=7) of the control group. Also, 15% (n=6) of the study group and 10% (n=4) of the control group had the TT genotype.<br />According to our results, the genotype distribution and allele prevalence were significantly different between the groups.https://www.pmjournal.ir/article_243876_d07eceee70ea54c39558f7556691adea.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320TERT Promoter Polymorphisms and Risk of Cervical Cancer121424387710.22034/pmj.2021.243877ENAida EtemadiResearch Center for cancer epidemiology and screening.
Aja University of Medical Sciences, Tehran, Iran.Maryam HassanlouFaculty of Biology, Women's university of semnan (Farzanegan), Semnan, IranJournal Article20201124TERT It has been shown that <em>TERT</em> is overexpressed in 90% of human cancers, and genetic alterations in the proximal promoter of TERT are significantly associated with a variety of different cancer types. In recent years, a new mechanism of <em>TERT</em> regulation through the non-coding driver mutations (C228T and C250T) in the <em>TERT</em> promoter has been reported in several cancer types. In the present study, we investigated the relationship between the Single Nucleotide Polymorphism (SNP) rs2853669 and cervical cancer.<br />The study included 80 individuals, including 50 patients with cervical cancer and 30 healthy controls. The samples from the participants underwent sequencing and genotyping using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method.<br />It was found that 16%, 24%, and 60% of the cervical cancer samples had the genotypes of AA, AG, and GG, respectively. In the control group, the frequencies were 13.33%, 50%, and 36.66% of the samples for the genotypes of AA, AG, and GG, respectively. <br />According to our findings, there was a significant association between the recessive model GG vs. AA+AG and cervical cancer susceptibility.<br /> https://www.pmjournal.ir/article_243877_b55ff4e32c5a110246964827e30ed559.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320Detection and Genotyping of HPV Infection Using a New Method Based on Real-Time PCR151724387910.22034/pmj.2021.243879ENAbbas ArdalanDepartment of biology, Faculty of science, Arak university, Arak, IranVahidreza EsfahaniDepartment of Cellular and Molecular Tehran Medical Sciences Branch,lslamic Azad UniversityJournal Article20201214The causalGiven the known relationship between the HPV infection and some malignancies, it is critical to develop methods for quick detection and quantitation of certain HPV types while encountering a suspected lesion. Early HPV detection is greatly important in monitoring and treating the disease development and progression. Detection of the viral DNA using PCR is the standard, noninvasive method for detecting cervical HPV infection. In the present study, we intended to develop a TaqMan genotyping assay that targets two types of high-risk HPV types (HPV 16 & 18) and two of the low-risk types (6 & 11).<br />The study included 75 samples positive for HPV, of which 37 were positive for HPV types 16 and 18, while 38 were positive for HPV types 6 and 11. The samples had been confirmed by a reference kit before. The samples underwent real-time PCR. Each reaction consisted of the 1X CAPITAL™ qPCR Probe Master Mix, specific primer pairs for HPV, and fluorescent-tagged probes.<br />According to our findings, all the samples genotyped using this method were compatible with the results by the reference kit, which was remarkable.<br />In conclusion, our type-specific approach based on real-time PCR could detect the entire samples positive for four types of HPV.https://www.pmjournal.ir/article_243879_94c63c5b610ba4e85f55ac729d178964.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210320Expression Evaluation of Long Non-Coding RNA TLC6 in Renal Cell Carcinoma182024388010.22034/pmj.2021.243880ENHamed NaghoosiResearch Center for Cancer Epidemiology and Screening, Aja University of Medical Sciences, Tehran, Iran and Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, IranSomaye ArabzadehDepartment of molecular and cellular biology, Faculty of Basic Science, Ale-Taha Institute of Higher Education, Tehran, IranJournal Article20201202Renal Cell Carcinomas (RCC) are a wide range of heterogeneous tumors that mainly originate in renal tubular epithelial cells. These carcinomas are more common in males older than 60. Studies have shown that overexpression of the Long Non-Coding RNA (lncRNA) TCL6, which is a lncRNA with roles in T-cell lymphoproliferative diseases, could suppress the proliferation and growth of Clear Cell RCC (ccRCC) cells. The present study intended to investigate the association of this specific lncRNA with ccRCC by comparing the expression levels in cancerous tissues with the adjacent normal tissues.<br />The study included 44 samples of ccRCC cells and healthy, adjacent tissue from the affected patients. The expression levels of TCL6 lncRNA were assessed in the cancerous tissues and the adjacent normal tissues using real-time PCR.<br />According to the results, TCL6 lncRNA was significantly underexpressed in the cancerous tissues than the adjacent normal tissues.<br />In conclusion, these findings indicated that TCL6 might have roles in the tumorigenesis regulation and development of RCC.https://www.pmjournal.ir/article_243880_5578c623cb589e81171f7ca8893e73e8.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553862020210301Apoptosis-Inducing Effect of Hesperidin on Breast Cancer Cell Line MCF7212324388110.22034/pmj.2021.243881ENSohameh MohebbiDepartment of Biotechnology, Faculty of Basic Science, Ale-Taha Institute of Higher Education, Tehran, Iran.Nafise PoorhasanDepartment of Cellular and Molecular, Faculty of Life Sciences, North Tehran Branch, Islamic Azad University, Faculty of Biological Sciences, Tehran, IranJournal Article20201229Hesperidin is a flavanone present in citrus fruits, such as oranges and lemons. It exerts non-toxic activities in normal cells; however, it has been reported to suppress cell proliferation in several cancer types. Moreover, it was shown that dietary hesperidin acts as an anti-carcinogenic agent for some tumors. In the present study, we investigated the effect of hesperidin on breast cancer cell line MCF7 and also its effects on the expression of apoptosis-related genes in this cell line.<br />MCF7 cells were divided into 4 groups, including 3 study groups and 1 control group. Each study group was treated with 50, 75, or 100 μg/mL hesperidin, while the control group was left untreated. The samples underwent real-time PCR using the primers specific for <em>BCL-2</em> and <em>BAX</em>, as the study genes, while GAPDH was used as the control.<br />According to our findings, hesperidin caused a significant decrease in BCL-2 mRNA levels at all the doses used in the study groups compared to the control group (p < 0.002). The observed decrease was dose-dependent. Also, hesperidin induced a significant overexpression of <em>BAX</em> when used in doses of 75 and 100 µg/mL in comparison to the control group.<br />The present study proved the significant apoptosis-inducing effect of hesperidin on the breast cancer cell line MCF7.https://www.pmjournal.ir/article_243881_89130816be2cca077f7b9544a198bb29.pdf