AmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Analysis of EGFR gene mutations in tissue samples of lung cancer tumors144345110.22034/pmj.2020.43451ENBlnd Ibrahim MohammedBiology Department, College of Science, Salahaddin University-Erbil, Erbil, IraqAmir MohammadiDepartment of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, MazandaranNafise PoorhasanPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726Lung cancer is the leading cause of cancer deaths worldwide. Approximately 25% of nonsmall-cell lung cancers have mutations in the<em> EGFR </em>gene, most of which occur in hotspot regions in exons 18, 19, 20, and 21. In-frame deletions in exon 19 (~50%) and the L858R point mutation in exon 21 (~40%) are associated with a favorable response to EGFR tyrosine kinase inhibitors. In this study, mutations of two exons of 19 and 21 in 50 lung cancer tumor samples were investigated by the sequence method. From 50 lung cancer patients, 8 (16%) patients had an L858R (c.2573T>G) mutation, 6 (12%) patients had deletion type 1a mutation, and one patient had deletion type 1b mutation. Examining the sequence of candidate genes associated with lung cancer can be very important in choosing the right treatment approach.https://www.pmjournal.ir/article_43451_3b7ae99e366ff787db6293d16a55b0ae.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Comparison of different methods of DNA extraction from paraffin-embedded tissues584345210.22034/pmj.2020.43452ENRamadhan IbrahimFish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University, Erbil, IraqSaeed MegdadiDepartment of biology, Nourdanesh institute of higher education, Meymeh, Isfahan, IranSareh Bakhshandeh BavarsadDepartment of Public Management, Faculty of Management, University of Shahrekord, IranNajme ShojaeiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726The most common human archival specimens are formalin-fixed and paraffin-embedded tissues. PCR-based techniques have been coupled with new developments in the extraction of DNA from FFPE. Herein, we report the results of a comparison of different methods of DNA extraction from FFPE specimens, including phenol-chloroform, salting-out, and silica-based commercial kits. Results showed no significant differences between the amounts of DNA obtained from each of the extraction methods studied; however, the salting-out DNA extraction method described is much easier and less toxic than the phenol–chloroform method.https://www.pmjournal.ir/article_43452_f4334212cbd8e556ab18d048f5e9609f.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis9114345310.22034/pmj.2020.43453ENFawziah M. MohammedFaculty of Allied Health Sciences KuwaitnMedical Laboratory SciencesHossein PakzadDepartment of biology, Tehran-east branch, Islamic azad university, Tehran, IranVahid Reza EsfahaniPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.https://www.pmjournal.ir/article_43453_696926aea6f2fdb675a0c9c98112c933.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Increased expression of the lnc H19 gene in the plasma of people with breast cancer12144345410.22034/pmj.2020.43454ENKazhaleh MohammadiResearcher and lecture assistant at the Medical Laboratory Science Department, Knowledge University, Kurdistan Region, Erbil, IraqSadegh Shojaei BaghiniPlant Biotechnology, Agriculture Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, IranMohammad Ali SaremiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726The majority of ncRNAs are known as long non-coding RNAs (lncRNAs) whose length exceeds 200 nucleotides. H19, a lncRNA, is the transcription product of the H19 gene, an oncogene in breast cancer, and is highly expressed in cancer tissues compared with normal tissues. The expression level of H19 is associated with the oncogenesis, proliferation, invasion, metastasis, and drug resistance of breast cancer. H19 expression levels were detected in breast cancer plasma using qRT-Real-Time PCR assay in 50 breast cancer samples and 50 healthy control samples. The results showed that the expression of this gene in both the tissue and the plasma of patients increased compared to that of healthy individuals.https://www.pmjournal.ir/article_43454_4a6cbd9219c6fd9f7ae988955f8df29e.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601The effect of silver nanoparticles on MCF7 breast cancer cell15174345510.22034/pmj.2020.43455ENAbdulkarim KarimCollege of Science, SALAHADDIN UNIVERSITY-ERBIL (SUE)Mohammad Ali Keshavarz ShahbazHuman Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, IranAfarin KomamPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726Currently, little is known about the mechanism(s) of AgNP-induced toxicity. Many previous studies, however, have provided strong evidence for a link between the AgNP-mediated production of ROS and the subsequent generation of oxidative stress. In the current study, the effects of Ag nanoparticles on the MCF-7 breast cell line were examined, and the biomarkers related to stress oxidative including GSH, superoxide dismutase, catalase, and ROS generation were evaluated. The results showed that Ag nanoparticles induced intracellular ROS generation in a dose- and time-dependent manner. Therefore, various studies should be performed to investigate the toxic effects of this substance on different cells.https://www.pmjournal.ir/article_43455_b8b4e4ceea546eb4eefe420c7731b7c7.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Relationship between PAI1 promoter 4G/5g polymorphism and stroke18204345610.22034/pmj.2020.43456ENSahar HassannejadResearcher and lecture assistant at the Medical Laboratory Science Department, Knowledge University, Kurdistan Region, Erbil, IraqEhsan RazeghianHuman Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, IranNajme ShojaeiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726PAI-1 has become recognized as a central molecule linking pathogenesis and progression of thrombotic vascular events, including stroke. Clinical and experimental studies show that PAI-1 deficiencies cause accelerated fibrinolysis and bleeding, whereas elevated PAI-1 plasma levels are associated with vascular thrombosis. Raised PAI1 plasma levels are related to a 1-bp guanine deletion/insertion (4G/5G) polymorphism in the promoter of the PAI1 gene. The 4G allele is associated with higher plasma PAI1 transcription and activity. In the current study, the association of higher PAI-1 plasma levels and the prevalence of the 4G/5G polymorphism in the PAI-1 gene promoter region in young patients with stroke were explored. Significantly, higher PAI-1 levels were observed in patients when compared to controls (<em>p </em>=002). The 4G/5G polymorphisms were significantly associated with increased PAI-1 levels, with the variant homozygous 4G/4G corresponding to mean values in patients versus controls.https://www.pmjournal.ir/article_43456_aede7816e139e7092a1cb533144f578b.pdfAmitisGen TECH Dev GroupPersonalized Medicine Journal2476-553851720200601Investigating the relationship between VEGF gene C936T-rs3025039 polymorphism and type 2 diabetes21244345710.22034/pmj.2020.43457ENHadi YariHuman Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran.Niloofar JAHANGIR SOOLTANIDepartment of Medical
Biotechnology, National Institute
of Genetic Engineering and
Biotechnology, Tehran, Iran.Mohammad Ali SaremiPersonalized Medicine Research Center of AmitisGen, Tehran, IranJournal Article20200726Type 2 diabetes (T2D), formerly known as adult-onset diabetes, is characterized by high blood sugar, insulin resistance, and relative lack of insulin.Diabetic retinopathy, a secondary microvascular complication of diabetes mellitus, is the leading cause of blindness. There is extensive evidence that the pathologic ocular angiogenesis in diabetic retinopathy is regulated by the vascular endothelial growth factor-A (VEGF-A).The single nucleotide polymorphism (SNP) C936T (rs3025039) of the VEGF gene has been investigated in relation to cancer, endometriosis, and age-related macular degeneration. The relationship between the rs3025039 VEGF gene polymorphism and the risk of type 2 diabetic retinopathy in 80 DT2 patients was examined. No significant association was found between polymorphism C936T and type 2 diabetes. It is recommended that this study be repeated on a larger population.https://www.pmjournal.ir/article_43457_ac7f4367c28e6823ab33c2e079041962.pdf