Introduction: Recent scientific promotion reveals that medicine is currently at a transition stage from programmatic to personalized handling on in infectious diseases. Methods: Herrien, this research was performed to evaluation seropositivity for L. monocytogenes based on personalized medicine among the women with and without annals of miscarriage by indirect immunofluorescence test (IIFA). Moreover, the relationship of seropositivity with demographic factors was also investigated. Results: Our outcome showed that 26.92% (35 cases) of women with an involuntary miscarriage were seropositive for L. monocytogenes, while 11% (11 cases) of healthy pregnant women were found positive for L. monocytogenes (P = 0.001). The most case of listeria has been found in age group of 30-35 years old, but no notable difference was found among different groups (P = 0.245). Moreover, there was a considerable association between listeria occurrence and history of abortion when compared with healthy pregnant women (P = 0.001). In addition, early abortion and the number of pregnancy were significantly linked to Listeria- seropositive in patients with spontaneous abortion (P = 0.041; P = 0.034). Conclusions: Further researches are required to appraise the clinical importance of L. monocytogenesin in pregnant women with symptomatic and asymptomatic infection related to personalized medicine.