Document Type : Original Article


1 Kermanshah University of Medical Sciences, School of the Medicine, Kermanshah, Iran

2 Personalized Medicine Research Center of AmitisGen, Tehran, Iran


Cervical cancer is the most widely screened cancer in the world both in high- and middle-income countries. HPV has been implicated in 99.7% of cervical squamous cell cancer cases worldwide. Viral E6 and E7 genes expression leads to alterations of the cellular genome integrity, including structural and numerical chromosomal instability resulting in chromosomal mis-segregation and aneuploidy. Centromere protein M (CENPM) is an essential centromere component for chromosome separation. In this study, we evaluated the expression of this gene at mRNA level by qRT-PCR method on 20 cancer samples who previously had feather papillomavirus infection, The results show a significant increase in the expression of this gene in cancer samples.


1. Smith RA, Andrews KS, Brooks D, Fedewa SA, ManassaramBaptiste D, Saslow D and Wender RC: Cancer screening in the 
United States, 2019: A review of current American cancer society 
guidelines and current issues in cancer screening. CA Cancer J 
Clin. 69:184–210. 2019.
2. Vuong D, Simpson PT, Green B, Cummings MC and Lakhani 
SR: Molecular classification of breast cancer. Virchows Arch. 
465:1–14. 2014.
3. Li G, Cottier M, Sabido O, Gentil-Perret A, Lambert C, 
Passebosc-Faure K, Genin C and Tostain J: The in vivo DNA 
aneuploidization during expansion of conventional carcinoma. In 
vivo. 16:341–344. 2002. 
4. Wu Q, Li B, Liu L and Sun S and Sun S: Centrosome 
dysfunction: A link between senescence and tumor immunity. 
Signal Transduct Target Ther. 5:1072020.
5. Howman EV, Fowler KJ, Newson AJ, Redward S, MacDonald 
AC, Kalitsis P and Choo KH: Early disruption of centromeric 
chromatin organization in centromere protein A (Cenpa) null 
mice. Proc Natl Acad Sci USA. 97:1148–1153. 2000.
6. Meluh PB and Koshland D: Evidence that the MIF2 gene of 
saccharomyces cerevisiae encodes a centromere protein with 
homology to the mammalian centromere protein CENP-C. Mol 
Biol Cell. 6:793–807. 1995.7. Liu ST, Hittle JC, Jablonski SA, Campbell MS, Yoda K and Yen 
TJ: Human CENP-I specifies localization of CENP-F, MAD1 and 
MAD2 to kinetochores and is essential for mitosis. Nat Cell Biol. 
5:341–345. 2003.
8. Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi 
T, Hiraoka Y, Sugata N, Todokoro K, Brown W and Ikemura T: 
CENP-H, a constitutive centromere component, is required for 
centromere targeting of CENP-C in vertebrate cells. EMBO J. 
20:4603–4617. 2001.
9. Basilico F, Maffini S, Weir JR, Prumbaum D, Rojas AM, 
Zimniak T, De Antoni A, Jeganathan S, Voss B, van Gerwen S, 
et al: The pseudo GTPase CENP-M drives human kinetochore 
assembly. eLife. 3:e029782014.
10. Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR III and 
Cleveland DW: The human CENP-A centromeric nucleosomeassociated complex. Nat Cell Biol. 8:458–469. 2006.
11. Chen J, Wu F, Shi Y, Yang D, Xu M, Lai Y and Liu Y: 
Identification of key candidate genes involved in melanoma 
metastasis. Mol Med Rep. 20:903–914. 2019. 
12. Kim WT, Seo SP, Byun YJ, Kang HW, Kim YJ, Lee SC, 
Jeong P, Song HJ, Choe SY, Kim DJ, et al: The anticancer effects 
of garlic extracts on bladder cancer compared to cisplatin: A 
common mechanism of action via centromere protein M. Am J 
Chin Med. 46:689–705. 2018.
13. Yu Z, Wang R, Chen F, Wang J and Huang X: Five novel 
oncogenic signatures could be utilized as AFP-related diagnostic 
biomarkers for hepatocellular carcinoma based on next-generation 
sequencing. Dig Dis Sci. 63:945–957. 2018 
14. Prystowsky MB, Adomako A, Smith RV, Kawachi N, 
McKimpson W, Atadja P, Chen Q, Schlecht NF, Parish JL, Childs 
G and Belbin TJ: The histone deacetylase inhibitor LBH589 
inhibits expression of mitotic genes causing G2/M arrest and 
cell death in head and neck squamous cell carcinoma cell lines. J 
Pathol. 218:467–477. 2009 
15. Huang PJ, Chiu LY, Lee CC, Yeh YM, Huang KY, Chiu CH 
and Tang P: mSignatureDB: A database for deciphering mutational 
signatures in human cancers. Nucleic Acids Res. 46(D1):D964–
D970. 2018.
16. Forbes SA, Beare D, Boutselakis H, Bamford S, Bindal N, 
Tate J, Cole CG, Ward S, Dawson E, Ponting L, et al: COSMIC: 
Somatic cancer genetics at high-resolution. Nucleic Acids Res. 
45(D1):D777–D783. 2017 
17. Rhodes DR, Kalyana-Sundaram S, Mahavisno V, Varambally 
R, Yu J, Briggs BB, Barrette TR, Anstet MJ, Kincead-Beal C, 
Kulkarni P, et al: Oncomine 3.0: Genes, pathways, and networks in 
a collection of 18,000 cancer gene expression profiles. Neoplasia. 
9:166–180. 2007.
18. Tang Z, Li C, Kang B, Gao G, Li C andrenal cell Zhang Z: 
GEPIA: A web server for cancer and normal gene expression 
profiling and interactive analyses. Nucleic Acids Res. 
45(W1):W98–W102. 2017.