Document Type : Review Article
1 Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2 Department of Cell and Molecular Biology, Faculty of Biological Sciences and Technology, Shahid Beheshti University G.C., Tehran, Iran
3 Department of Biotechnology, Faculty of Basic Science, Ale-Taha Institute of Higher Education, Tehran, Iran.
In this article, the features of DNA-functionalized goldnanoparticles (GNRs), including the size-dependent color, the amount of conjugated DNA, and the fluorescence quenchers will be described. DNA and aptamer conjugated GNRs can be applied for producing the colorimetric and fluorescent biosensors to detect all types of disease markers including DNA, RNA, protein and other small molecular metabolites.
The early phase of this work is performed in clean buffers and serum samples. DNA-conjugated GNRs delivery into the cells is recently used for intracellular diagnosis in personalized medicine. Simultaneously, DNA-functionalized GNRs can be used to deliver the antisense DNA for gene therapy applications.
With targeting both diagnosis and treatment applications, DNA- functionalized GNRs can be used as a suitable approach to reach theranostics purposes (diagnosis and treatment in a simultaneous manner).