Document Type : Original Article
Authors
1 National Institute of Genetic Engineering and Biotechnology
2 Department of Genetics, Reproductive Biomedicine research center, Royan Institute of Reproductive Biomedicine, ACECR, Tehran, Iran
3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran
Abstract
Obesity and type 2 diabetes are two complex disorders with strong genetic backgrounds, and both disorders cause other diseases and increase mortality. FTO is a protein-coding gene located on the long arm of chromosome 16 and linked to type 2 diabetes and obesity. The current study investigated the relationship between the RS16953002 polymorphism gene and type 2 diabetes and obesity in a population from Tehran. The study population included 150 people with type 2 diabetes and 150 healthy individuals. Genotyping was performed by RFLP-PCR. The results indicated the presence of the GG allele in 81% of diabetic patients but in only64% of non-diabetic participants, which shows a statistically significant difference in this regard. Thus, the current study has shown the role of FTO gene polymorphisms in the pathogenesis of people with type 2 diabetes and obesity.
Keywords
RM, Lindgren CM, et al. A common variant in the FTO gene is
associated with body mass index and predisposes to childhood
and adult obesity. Science. 2007;316(5826):889-94.
3. Cha SW, Choi SM, Kim KS, Park BL, Kim JR, Kim JY, et al.
Replication of genetic effects of FTO polymorphisms on BMI in a
Korean population. Obesity (Silver Spring). 2008;16(9):2187-9..
4. Gerken T, Girard CA, Tung YC, Webby CJ, Saudek V,
Hewitson KS, et al. The obesity-associated FTO gene encodes
a 2-oxoglutarate-dependent nucleic acid demethylase. Science.
2007;318(5855):1469-72.
5. Scuteri A, Sanna S, Chen WM, Uda M, Albai G, Strait J, et
al. Genome-wide association scan shows genetic variants in the
FTO gene are associated with obesityrelated traits. PLoS genetics.
2007; 3:1200–1210..
6. Dina C, Meyre D, Gallina S, Durand E, Korner A, Jacobson P,
et al. Variation in FTO contributes to childhood obesity and severe
adult obesity. Nature genetics. 2007;39(6):724-6.
7. Hinney A, Nguyen TT, Scherag A, Friedel S, Bronner G, Muller
TD, et al. Genome wide association (GWA) study for early onset
extreme obesity supports the role of fat mass and obesity associated
gene (FTO) variants. PloS one. 2007;2. 8. Peeters A, Beckers S,
Verrijken A, Roevens P, Peeters P, Van Gaal L, et al. Variants in
the FTO gene are associated with common obesity in the Belgian
population. Molecular genetics and metabolism. 2008;93(4):481-4.
8. Hotta K, Nakata Y, Matsuo T, Kamohara S, Kotani K,
Komatsu R, et al. Variations in the FTO gene are associated
with severe obesity in the Japanese. Journal of human genetics.
2008;53(6):546-53..
9. Marvelle AF, Lange LA, Qin L, Adair LS, Mohlke KL.
Association of FTO with obesity-related traits in the Cebu
Longitudinal Health and Nutrition Survey (CLHNS) Cohort
Diabetes. 2008;57(7):1987-91.
10. Ng MC, Park KS, Oh B, Tam CH, Cho YM, Shin HD, et al.
Implication of genetic variants near TCF7L2, SLC30A8, HHEX,
CDKAL1, CDKN2A/B, IGF2BP2, and FTO in type 2 diabetes
and obesity in 6,719 Asians. Diabetes. 2008;57(8):2226-33..
11. Chang YC, Liu PH, Lee WJ, Chang TJ, Jiang YD, Li HY, et
al. Common variation in the fat mass and obesityassociated (FTO)
gene confers risk of obesity and modulates BMI in the Chinese
population. Diabetes. 2008;57(8):2245-52.
12. Tan JT, Dorajoo R, Seielstad M, Sim XL, Ong RT, Chia KS,
et al. FTO variants are associated with obesity in the Chinese and
Malay populations in Singapore. Diabetes. 2008;57(10):2851-7.
13. Grant SF, Li M, Bradfield JP, Kim CE, Annaiah K, Santa
E, et al. Association analysis of the FTO gene with obesity in
children of Caucasian and African ancestry reveals a common
tagging SNP. PloS one. 2008;3.
14. Ohashi J, Naka I, Kimura R, Natsuhara K, Yamauchi T,
Furusawa T, et al. FTO polymorphisms in oceanic populations.
Journal of human genetics. 2007;52(12):1031-5.
15. Horikoshi M, Hara K, Ito C, Shojima N, Nagai R, Ueki K,
et al. Variations in the HHEX gene are associated with increased
risk of type 2 diabetes in the Japanese population. Diabetologia.
2007;50(12):2461-6.
16. Li H, Wu Y, Loos RJ, Hu FB, Liu Y, Wang J, et al. Variants
in the fat mass- and obesity-associated (FTO) gene are not
associated with obesity in a Chinese Han population. Diabetes.
2008;57(1):264-8
)