Document Type : Original Article


1 Researcher and lecture assistant at the Medical Laboratory Science Department, Knowledge University, Kurdistan Region, Erbil, Iraq

2 Genome research center, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran.

3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

4 Plant Biotechnology, Agriculture Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran.


Spermatogenesis is a very complex process that can be affected by a number of factors which may lead to reduced fertility or infertility. Busulfan kills spermatogonia stem cells and disrupts the connections between Sertoli and spermatogonia cells at base layers. Moreover, vitamin D is very important in the reproductive system of men. In this study, the effect of vitamin D on biosulfur-induced azoospermia in mice was investigated. The results showed an increase in the quality of sperm parameters in the group treated with vitamin D.


1. Allouche L, Hamadouche M, Touabti A. Chronic effects of 
low lead levels on sperm quality, gonadotropins and testosterone 
in albino rats. Exp Toxicol Pathol. 2009 Sep; 61(5): 503-10. 
2. Shan G, Tang T, Zhang X. The protective effect of ascorbic 
acid and thiamine supplementation against damage caused by lead 
in the testes of mice. J Huazhong Univ Sci Technolog Med Sci. 009 Feb; 29(1): 68-72.
3. Graca A, Ramalho-Santos J, de Lourdes Pereira M. Effect of 
lead chloride on spermatogenesis and sperm parameter in mice. 
Asian J Androl. 2004 Sep; 6(3): 237-410. 
4. Sokol RZ. The effect of duration of exposure on the expression 
of lead toxicity on the male reproductive axis. J Androl. 1990 
Nov-Dec; 11(6):521-6
5. Anjum MR, Sainath SB, Suneetha Y, Reddy PS. Lead acetate 
induced reproductive and paternal mediated developmental 
toxicity in rats. Ecotoxicol Environ Saf. 2011 May; 74(4): 793-9
6. Golshan- Iranpoor F, Emami E. The effects of lead on motility, 
viability and DNA denaturation of cauda epididymal spermatozoa 
of mouse. J Shahrekord Univ Med Sci. 2011Oct-Nov; 13(4):1-8. 
[full text in Persian] 
7. Alvarez JA, Ashraf A. Role of vitamin d in insulin secretion 
and insulin sensitivity for glucose homeostasis. Int J Endocrinol. 
2010; 7(1):351-385.
8. Holick MF, Chen TC. Vitamin D deficiency: a worldwide 
problem with health consequences. Am J Clin Nutr. 2008 Apr; 
9. Sung CC, Liao MT, Lu KC, Wu CC. Role of vitamin D in 
insulin resistance. J Biomed Biotechnol. 2012 Sep; 2012:634195. 
10. Nimitphong H, Chanprasertyothin S, Jongjaroenprasert 
W, Ongphiphadhanakul B. The association between vitamin D 
status and circulating adiponectin independent of adiposity in 
subjects with abnormal glucose tolerance. Endocrine. 2009 Oct; 
11. Patel P, Poretsky L, Liao E. Lack of effect of subtherapeutic 
vitamin D treatment on glycemic and lipid parameters in Type 2 
diabetes: Apilot prospective randomized trial. J diabetes. 2010 
Mar; 2(1):36-40. 12- Foss YJ. Vitamin D deficiency is the cause 
of common obesity. Med Hypotheses. 2009 Mar; 7(2): 314–321.
12. Souri E, Farsam H, Hasani M, Azimi kheirabadi Z. 
Evaluation of antioxidant activity of 25 plant seeds used in Iranian 
folk medicine. JMP. 2003 Dec; 4(8):27-34.
13. Naziroğlu M. Enhanced testicular antioxidant capacity in 
streptozotocin-induced diabetic rats: protective role of vitamins 
C and E and selenium. Biol Trace Elem Res. 2003 Jul;94(1):61-72
14. Baghy Nia N, Oryan SH, Fani A, Maleky Rad A. The effect 
of Cardamom- tea watery extract on oxidative stress. AMUJ. 2008 
Dec; 11(4):1-7. [full text in Persian.