Personalized and Precision Medicine Journal
Where Innovations Meets Personalized and Precision Medicine

Evaluation of V617F JAK 2 gene mutation by high resolution Melting method in patient witherythrocytosis

Authors

Fawziah M. Mohammed 1
Hossein Pakzad 2
Vahid Reza Esfahani 3

1 Faculty of Allied Health Sciences KuwaitnMedical Laboratory Sciences

2 Department of biology, Tehran-east branch, Islamic azad university, Tehran, Iran

3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

https://doi.org/10.22034/pmj.2020.43453
Abstract
Although several techniques have been developed for the detection of JAK2 V617F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK2 V617F mutation by the high-resolution melting method in 20 patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed 100% sensitivity and 100% positive predictive value for this methodology in the patient sample set tested.

Keywords

Myeloproliferative
JAK2
point mutation
High-Resolution Melting
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Personalized and Precision Medicine Journal
Volume 5, Issue 17
Original article
Spring 2020
Pages 9-11

  • Receive Date 26 July 2020

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