Personalized and Precision Medicine Journal
Where Innovations Meets Personalized and Precision Medicine

The effect of silver nanoparticles on MCF7 breast cancer cell

Document Type : Original Article

Authors

Abdulkarim Karim 1
Mohammad Ali Keshavarz Shahbaz 2
Afarin Komam 3

1 College of Science, SALAHADDIN UNIVERSITY-ERBIL (SUE)

2 Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran

3 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

https://doi.org/10.22034/pmj.2020.43455
Abstract
Currently, little is known about the mechanism(s) of AgNP-induced toxicity. Many previous studies, however, have provided strong evidence for a link between the AgNP-mediated production of ROS and the subsequent generation of oxidative stress. In the current study, the effects of Ag nanoparticles on the MCF-7 breast cell line were examined, and the biomarkers related to stress oxidative including GSH, superoxide dismutase, catalase, and ROS generation were evaluated. The results showed that Ag nanoparticles induced intracellular ROS generation in a dose- and time-dependent manner. Therefore, various studies should be performed to investigate the toxic effects of this substance on different cells.

Keywords

Ag nanoparticle
MCF-7 cell line
stress oxidative
ROS
  1. Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ, Hong Y. 2008. DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol. Appl. Pharmacol. 233: 404–410.
  2. Ahamed M, Posgai R, Gorey TJ, Nielsen M, Hussain SM, Rowe JJ. 2010. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol. Appl. Pharmacol. 242: 263–269.
  3. Choi O, Hu Z. 2008. Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ. Sci. Technol. 42: 4583–4588.
  4. Farkas J, Christian P, Gallego-Urrea JA, Roos N, Hassellov M, Tollefsen KE,

Thomas KV. 2010. Effects of silver and gold nanoparticles on rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat. Toxicol. 96: 44–52.

  1. Ringwood AH, McCarthy M, Bates TC, Carroll DL. 2010. The effects of silver nanoparticles on oyster embryos. Mar. Environ. Res. 69: S49–51.
  2. Li PW, Kuo TH, Chang JH, Yeh JM, Chan WH. 2010. Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles. Toxicol. Lett. 197:82–87.
  3. Kim S, Choi JE, Choi J, Chung KH, Park K, Yi J, Ryu DY. 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol. In Vitro 23: 1076–1084
  4. Kim S, Choi JE, Choi J, Chung KH, Park K, Yi J, Ryu DY. 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol. In Vitro 23: 1076–1084
  5. Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI, Wiesner MR, Nel AE. 2006. Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett. 6: 1794–1807.
  6. Wei L, Lu J, Xu H, Patel A, Chen ZS, Chen G. Silver nanoparticles: synthesis, properties, and therapeutic applications. Drug Discov Today. 2015; 20: 595-601.
  7. Raghunandan D, Ravishankar B, Sharanbasava G, et al. Anti-cancer studies of noble metal nanoparticles synthesized using different plant extracts. Cancer Nanotechnol 2011; 2: 57-65.
  8. Gurunathan S, Raman J, Malek SNA, John PA, Vikineswary S. Green synthesis of silver nanoparticles using Ganoderma neojaponicum Imazeki: A potential cytotoxic agent against breast cancer cells.Int JNanomedicinn 2013;8:4399-4413.

14.Nazir S, Hussain T, Iqbal Md, Mazhar K, Muazzam AG, Ismail J Md. Novel and costeffective green synthesis of silver nanoparticles and their in vivo antitumor

properties against humancancer cell lines. Biosci Tech 2011; 2: 425-443.

  1. Guo D, Zhu L, Huang Z, et al. Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions. Biomaterials 2013; 34: 7884-7894.

16.Kathiravan V, Ravi S, Ashokkumar S. Synthesis of silver nanoparticles from Melia dubia leaf extract and their in vitro anticancer activity. Spectrochim Acta A Mol Biomol Spectrosc 2014; 130: 116-121.

  1. Nagajyothi PC, Sreekanth TVM, Lee JI, Lee KD. Mycosynthesis: antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. J
Personalized and Precision Medicine Journal
Volume 5, Issue 17
Original article
Spring 2020
Pages 15-17

  • Receive Date 26 July 2020

Home

Publication Ethics

Guide for Authors

Submit Manuscript

Contact Us