Adjustment of a Fibrosis Marker, Pro-Inflammatory Cytokines, and IgE in Asthmatic Animals

Dalouchi, Fereshteh; Sharifi Aghdam, Zeynab; Falak, Raza; Bakhshesh, Morteza; Hajidazeh, Maryam; Naseroleslami, Maryam; Mehrab Mohseni, Mahdieh; Azizi, Yaser; Aboutaleb, Nahid

doi:10.22034/pmj.2023.2009908.1010

Document Type : Original Article

Authors

¹ Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.

² Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran

³ Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

⁴ Khomein University of Medical Sciences, Khomein, Iran

⁵ Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, &lrm;Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

⁶ Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

https://doi.org/10.22034/pmj.2023.2009908.1010

Abstract

Background: A lot of patients are suffering from asthma. For decreasing the asthma symptoms, we studied the effects of conditioned medium (CM) of human amniotic membrane mesenchymal stem cells (hAM-MSCs) as a source of anti-inflammatory cytokines on splenocyte and lung tissue of asthmatic Balb/c mice.
Methods: Forty mice were categorized into four groups; ovalbumin (OVA)-induced asthma, CM-treated asthma, DMEM (Dulbecco's Modified Eagle Medium)-treated asthma, and saline control. Each group received related treatment. The lung alpha-smooth muscle actin (α-SMA) and splenocyte inflammatory cytokines and IgE were examined through Western blot analysis.
Results: Western blot showed α-SMA overexpression in the OVA and DMEM groups compared with the saline group. CM therapy could significantly reverse it compared with OVA and OVA+DMEM categories by elevating IL-10 and IFN-γ and reducing IL-4, IgE, and TGF-β .
Conclusion: CM treatment could improve asthma symptoms by adjusting α-SMA in lung tissue and pro-inflammatory cytokines and IgE in splenocytes.

Keywords

20.1001.1.27173860.2023.8.30.5.4

Main Subjects

Biotechnology

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Personalized Medicine Journal

Adjustment of a Fibrosis Marker, Pro-Inflammatory Cytokines, and IgE in Asthmatic Animals

References

References

Volume 8, Issue 30 - Serial Number 30
original article
September 2023
Pages 32-40

Adjustment of a Fibrosis Marker, Pro-Inflammatory Cytokines, and IgE in Asthmatic Animals

References

References

Volume 8, Issue 30 - Serial Number 30 original articleSeptember 2023Pages 32-40

Volume 8, Issue 30 - Serial Number 30
original article
September 2023
Pages 32-40