Personalized and Precision Medicine Journal
Where Innovations Meets Personalized and Precision Medicine

Targeted Screening of Membrane Proteins of Haemophilus Ducreyi with the Aim of Drug Targets Identification

Document Type : Original Article

Authors

Sahar Khorsand-Dehkordi 1
Farnoosh Honarmand 2
Zahra Ahmadzadeh Chaleshtori 3

1 Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

2 Department of Biology, Faculty of Basic Sciences, Tehran Branch, University of Science and Culture, Tehran, Iran.

3 Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

https://doi.org/10.22034/pmj.2024.2024174.1032
Abstract
Chancroid is an STI characterized by the Gram-negative bacteria Haemophilus ducreyi. Controlling chancroid is challenging, and the primary treatment accessible is antimicrobial therapy. However, drug resistance has been seen in places where the disease is common. Due to recent global outbreaks of sexually transmitted infections (STIs), it is crucial to continue researchi ng innovative treatment options and prevention measures. We used reverse vaccinology and subtraction genomic methods to determine potential vaccination and therapeutic targets against H. ducreyi in silico. We found 56 Secreted proteins, with 159 membrane molecules and 515 cytoplasmic proteins. We assessed their need, operation, and ability to cause disease. We identified 6 potential vaccination targets and three pharmacological targets inside pathogenicity islands. The discovered targets may be utilized in future initiatives to manage chancroid globally.

Keywords

Sexually transmitted infections
Chancroid
H. ducreyi
Reverse vaccinology

Subjects

  1. de Sarom A, Kumar Jaiswal A, Tiwari S, de Castro Oliveira L, Barh D, Azevedo V, Jose Oliveira C, de Castro Soares S. Putative vaccine candidates and drug targets identified by reverse vaccinology and subtractive genomics approaches to control Haemophilus ducreyi, the causative agent of chancroid. Journal of The Royal Society Interface. 2018 May 31;15(142):20180032.
  2. Lopez AD, Mathers CD. Measuring the global burden of disease and epidemiological transitions: 2002–2030. Annals of Tropical Medicine & Parasitology. 2006 Aug 1;100(5-6):481-99.
  3. Loh AJ, Ting EL, Wi TE, Mayaud P, Chow EP, Santesso N, Falconer J, Ofori-Asenso R, Ong JJ. The diagnostic accuracy of syndromic management for genital ulcer disease: a systematic review and meta-analysis. Frontiers in Medicine. 2022 Jan 3;8:806605.
  4. Parija SC. Haemophilus and Bordetella. InTextbook of Microbiology and Immunology 2023 Mar 17 (pp. 579-596). Singapore: Springer Nature Singapore.
  5. Martens EC, Neumann M, Desai MS. Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier. Nature Reviews Microbiology. 2018 Aug;16(8):457-70.
  6. Piri Gharaghie T, Doosti A, Mirzaei SA. Prevalence and antibiotic resistance pattern of Acinetobacter spp. infections in Shahrekord medical centers. Developmental Biology. 2021 Nov 22;13(4):35-46.
  7. Piri-Gharaghie T. Polycystic ovary syndrome and genetic factors influencing its development: A review article. Personalized Medicine Journal. 2021 Dec 1;6(23):25-9.
  8. Ramdial PK. Dermatopathological challenges in the human immunodeficiency virus and acquired immunodeficiency syndrome era. Histopathology. 2010 Jan;56(1):39-56.
  9. González-Beiras C, Marks M, Chen CY, Roberts S, Mitjà O. Epidemiology of Haemophilus ducreyi Emerging infectious diseases. 2016 Jan;22(1):1.
  10. Trager JD. Sexually transmitted diseases causing genital lesions in adolescents. Adolescent Medicine Clinics. 2004 Jun 1;15(2):323.
  11. Leonard CA, Borel N. Chronic chlamydial diseases: from atherosclerosis to urogenital infections. Current Clinical Microbiology Reports. 2014 Dec;1:61-72.
  12. Ghajari G, Nabiuni M, Amini E. The association between testicular toxicity induced by Li2Co3 and protective effect of Ganoderma lucidum: Alteration of Bax & c-Kit genes expression. Tissue and Cell. 2021 Oct 1;72:101552.
  13. Yadollahi A, Ghajari G. Transgenic induction in Sesamum indicum with recombinant pBi121 expression construct containing CYP81Q1 and aroA genes using Agrobacterium tomfacensis.
  14. Wood GE, Dutro SM, Totten PA. Target cell range of Haemophilus ducreyi hemolysin and its involvement in invasion of human epithelial cells. Infection and immunity. 1999 Aug 1;67(8):3740-9.
  15. Brothwell JA, Griesenauer B, Chen L, Spinola SM. Interactions of the skin pathogen Haemophilus ducreyi with the human host. Frontiers in Immunology. 2021 Feb 3;11:615402.
  16. Cole LE, Toffer KL, Fulcher RA, San Mateo LR, Orndorff PE, Kawula TH. A humoral immune response confers protection against Haemophilus ducreyi Infection and immunity. 2003 Dec;71(12):6971-7.
  17. Cole LE, Toffer KL, Fulcher RA, San Mateo LR, Orndorff PE, Kawula TH. A humoral immune response confers protection against Haemophilus ducreyi Infection and immunity. 2003 Dec;71(12):6971-7.
  18. Chávez-Sánchez L, Espinosa-Luna JE, Chávez-Rueda K, Legorreta-Haquet MV, Montoya-Díaz E, Blanco-Favela F. Innate immune system cells in atherosclerosis. Archives of medical research. 2014 Jan 1;45(1):1-4.
  19. Ghourchian H, Pecho RD, Karimi-Dehkordi M, Mazandarani A, Ghajari G, Piri-Gharaghie T. Novel Niosome-Encapsulated 2, 5-Diketopiperazine (BHPPD): Synthesis, Formulation, and Anti-breast Cancer Activity. Applied Biochemistry and Biotechnology. 2023 Aug 25:1-22.
  20. Rac H, Gould AP, Eiland LS, Griffin B, McLaughlin M, Stover KR, Bland CM, Bookstaver PB. Common bacterial and viral infections: review of management in the pregnant patient. Annals of Pharmacotherapy. 2019 Jun;53(6):639-51.
  21. Piri-Gharaghie T, Ghajari G, Tolou-Shikhzadeh-Yazdi S, Aghassizadeh-Sherbaf M, Khorsand-Dehkordi S. Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics. Scientific Reports. 2023 Jul 10;13(1):11116.
  22. Marks M, Mitjà O, Vestergaard LS, Pillay A, Knauf S, Chen CY, Bassat Q, Martin DL, Fegan D, Taleo F, Kool J. Challenges and key research questions for yaws eradication. The Lancet infectious diseases. 2015 Oct 1;15(10):1220-5.
  23. Lewis DA. Epidemiology, clinical features, diagnosis and treatment of Haemophilus ducreyi–a disappearing pathogen?. Expert review of anti-infective therapy. 2014 Jun 1;12(6):687-96.
  24. Avershina E, Shapovalova V, Shipulin G. Fighting antibiotic resistance in hospital-acquired infections: current state and emerging technologies in disease prevention, diagnostics and therapy. Frontiers in microbiology. 2021 Jul 21;12:707330.
  25. Saeed Mohammed A, Ghajari G. Epithelial-Mesenchymal Rransition and its Role in Breast Cancer Metastasis. Personalized Medicine Journal. 2022 Mar 20;7(24):8-13.
  26. Piri-Gharaghie T, Doosti A, Mirzaei SA. Identification of antigenic properties of Acinetobacter baumannii proteins as novel putative vaccine candidates using reverse vaccinology approach. Applied Biochemistry and Biotechnology. 2022 Oct;194(10):4892-914.
  27. Rawal K, Sinha R, Abbasi BA, Chaudhary A, Nath SK, Kumari P, Preeti P, Saraf D, Singh S, Mishra K, Gupta P. Identification of vaccine targets in pathogens and design of a vaccine using computational approaches. Scientific reports. 2021 Sep 2;11(1):17626.
  28. Mesnage S, Dellarole M, Baxter NJ, Rouget JB, Dimitrov JD, Wang N, Fujimoto Y, Hounslow AM, Lacroix-Desmazes S, Fukase K, Foster SJ. Molecular basis for bacterial peptidoglycan recognition by LysM domains. Nature communications. 2014 Jun 30;5(1):4269.
  29. Dik DA, Marous DR, Fisher JF, Mobashery S. Lytic transglycosylases: concinnity in concision of the bacterial cell wall. Critical Reviews in Biochemistry and Molecular Biology. 2017 Sep 3;52(5):503-42.
  30. Chen YC, Kalawong R, Toyofuku M, Eberl L. The role of peptidoglycan hydrolases in the formation and toxicity of Pseudomonas aeruginosa membrane vesicles. MicroLife. 2022;3:uqac009.
  31. Dubnau D, Blokesch M. Mechanisms of DNA uptake by naturally competent bacteria. Annual review of genetics. 2019 Dec 3;53:217-37.
  32. Gobbetti M, De Angelis M, Di Cagno R, Minervini F, Limitone A. Cell–cell communication in food related bacteria. International journal of food microbiology. 2007 Nov 30;120(1-2):34-45.
  33. Fusco WG, Choudhary NR, Stewart SM, Alam SM, Sempowski GD, Elkins C, Leduc I. Defining potential vaccine targets of Haemophilus ducreyi trimeric autotransporter adhesin DsrA. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2015 Apr 1;34(2):73-82.
  34. Bauer ME, Fortney KR, Harrison A, Janowicz DM, Munson RS, Spinola SM. Identification of Haemophilus ducreyi genes expressed during human infection. Microbiology. 2008 Apr 1;154(4):1152-60.
  35. Gangaiah D, Zhang X, Baker B, Fortney KR, Gao H, Holley CL, Munson Jr RS, Liu Y, Spinola SM. Haemophilus ducreyi seeks alternative carbon sources and adapts to nutrient stress and anaerobiosis during experimental infection of human volunteers. Infection and Immunity. 2016 May;84(5):1514-25.

 

 

 

Personalized and Precision Medicine Journal
Volume 9, Issue 32 - Serial Number 32
Original article
Winter 2024
Pages 8-15

  • Receive Date 30 November 2023
  • Revise Date 15 January 2024
  • Accept Date 22 February 2024

Home

Publication Ethics

Guide for Authors

Submit Manuscript

Contact Us