Personalized and Precision Medicine Journal
Where Innovations Meets Personalized and Precision Medicine

Investigation of Antioxidant and Cytotoxic Effects of Cerium Oxide Nanoparticles Synthesized Using Aqueous Extract of Hyssopus Officinalis Plant on MDA-MB231 Breast Cancer Cell Line

Document Type : Original Article

Authors

Mahnaz Tourani 1
Kamran Eghbalpour 2
Nahid Eghbalpour 2
Ali Neamati 3

1 Departmant of biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

2 Departmant of biology, Shahre-e-Qods branch, Islamic Azad University, Tehran, Iran.

3 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

https://doi.org/10.22034/pmj.2024.2024143.1031
Abstract
Free radicals are naturally produced in the body and are inhibited by the body’s antioxidants. The excessive production of free radicals and the inability of the body to remove them lead to oxidative stress in the body, which can lead to many diseases, including cancer. Nanoparticles are compounds that have been given much attention to cancer prevention and treatment, due to their specific biological characteristics and their small size. This study aimed to evaluate the cytotoxic and antioxidant potential of serum oxide nanoparticles synthesized using aqueous extract of Hyssopus officinalis.
To perform the MTT assay, first, the MDA-MB231 cancerous cells were cultured, seeded and then treated for 24, 48 and 72 hours. Subsequently, MTT was performed and finally, absorption at 517 nm was recorded. The antioxidant potential of the CeO-NPs was evaluated by estimating the amount of ABTS and DPPH free radicals inhibiting in different concentrations of nanoparticles.
The results showed that the CeO-NPs were able to inhibit the ABTS and DPPH free radicals with a mean concentration (IC50) of about 62 and 31.2 μg / ml. Also, the CeO-NPs inhibited cancer cells with IC50 of about 400 µg/ml, 48 hours after exposure. According to the antioxidant results obtained from this paper, it is suggested that by performing further experiments, this nanoparticle can be used as an antioxidant supplement.

Keywords

Cerium Oxide Nanoparticle
Breast cancer
Antioxidant
Hyssopus officinalis extract

Subjects

  1. Fisher R, Pusztai L, Swanton C. Cancer heterogeneity: implications for targeted therapeutics. British journal of cancer. 2013;108(3):479-85.
  2. Fiaschi T, Chiarugi P. Oxidative stress, tumor microenvironment, and metabolic reprogramming: a diabolic liaison. International journal of cell biology. 2012;2012:762825.
  3. Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezaie A. Pesticides and oxidative stress: a review. Medical science monitor : international medical journal of experimental and clinical research. 2004;10(6):Ra141-7.
  4. Roome T, Dar A, Ali S, Naqvi S, Choudhary MIJJoe. A study on antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective actions of Aegiceras corniculatum (stem) extracts. 2008;118 3:514-21.
  5. Senapati KK, Roy S, Borgohain C, Phukan PJJoMCA-c. Palladium nanoparticle supported on cobalt ferrite: An efficient magnetically separable catalyst for ligand free Suzuki coupling. 2012;352:128-34.
  6. Narayanan KB, Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Advances in Colloid and Interface Science. 2010;156(1):1-13.
  7. Borovski T, De Sousa EMF, Vermeulen L, Medema JP. Cancer stem cell niche: the place to be. Cancer research. 2011;71(3):634-9.
  8. Faure C, Derré A, Neri W. Spontaneous Formation of Silver Nanoparticles in Multilamellar Vesicles. The Journal of Physical Chemistry B. 2003;107(20):4738-46.
  9. Zhang ZG, Zhang L, Croll SD, Chopp M. Angiopoietin-1 reduces cerebral blood vessel leakage and ischemic lesion volume after focal cerebral embolic ischemia in mice. Neuroscience. 2002;113(3):683-7.
  10. Wijnhoven SWP, Peijnenburg WJGM, Herberts CA, Hagens WI, Oomen AG, Heugens EHW, et al. Nano-silver – a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology. 2009;3(2):109-38.
  11. Sadeghi B, Rostami A, Momeni SS. Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2015;134:326-32.
  12. Celardo I, Pedersen JZ, Traversa E, Ghibelli L. Pharmacological potential of cerium oxide nanoparticles. Nanoscale. 2011;3(4):1411-20.
  13. Bondet V, Brand-Williams W, Berset CJL-FS, Technology. Kinetics and Mechanisms of Antioxidant Activity using the DPPH.Free Radical Method. 1997;30:609-15.
  14. Narayanan KB, Sakthivel N. Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface Sci. 2011;169(2):59-79.
  15. Park E-J, Choi J, Park YK, Park KJT. Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells. 2008;245 1-2:90-100.
  16. Lin W, Huang YW, Zhou XD, Ma Y. Toxicity of cerium oxide nanoparticles in human lung cancer cells. International journal of toxicology. 2006;25(6):451-7.
  17. Sukirtha R, Priyanka KM, Antony JJ, Kamalakkannan S, Thangam R, Gunasekaran P, et al. Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model. 2012;47:273-9.
  18. Venkatesan B, Subramanian V, Tumala A, Vellaichamy E. Rapid synthesis of biocompatible silver nanoparticles using aqueous extract of Rosa damascena petals and evaluation of their anticancer activity. Asian Pacific Journal of Tropical Medicine. 2014;7:S294-S300.
  19. Ranjitham AM, Suja RM, Tiwari SK, editors. INVITRO EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTICANCER ACTIVITIES AND CHARACTERISATION OF BRASSICA OLERACEA. VAR. BORTRYTIS. L SYNTHESIZED SILVER NANOPARTICLES2013.
  20. Nagajyothi PC, Sreekanth TV, Lee JI, Lee KD. Mycosynthesis: antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. Journal of photochemistry and photobiology B, Biology. 2014;130:299-304.
  21. Devanathadesikan Seshadri V, Vijayaraghavan P, Kim YO, Kim HJ, Ahmed Al-Ghamdi A, Elshikh MS, et al. In vitro antioxidant and cytotoxic activities of polyherbal extracts from Vetiveria zizanioides, Trichosanthes cucumerina, and Mollugo cerviana on HeLa and MCF-7 cell lines. Saudi Journal of Biological Sciences. 2020;27(6):1475-81.
  22. Suresh D, Shobharani RM, Nethravathi PC, Pavan Kumar MA, Nagabhushana H, Sharma SC. Artocarpus gomezianus aided green synthesis of ZnO nanoparticles: luminescence, photocatalytic and antioxidant properties. Spectrochimica acta Part A, Molecular and biomolecular spectroscopy. 2015;141:128-34.
  23. Hyung S-J, DeToma AS, Brender JR, Lee S, Vivekanandan S, Kochi A, et al. Insights into antiamyloidogenic properties of the green tea extract (-)-epigallocatechin-3-gallate toward metal-associated amyloid-β species. Proc Natl Acad Sci U S A. 2013;110(10):3743-8.
  24. Kim TI, McCall JG, Jung YH, Huang X, Siuda ER, Li Y, et al. Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science (New York, NY). 2013;340(6129):211-6.
Personalized and Precision Medicine Journal
Volume 9, Issue 32 - Serial Number 32
Original article
Winter 2024
Pages 36-41

  • Receive Date 27 November 2024
  • Revise Date 18 January 2024
  • Accept Date 01 March 2024

Home

Publication Ethics

Guide for Authors

Submit Manuscript

Contact Us