Personalized and Precision Medicine Journal

Abstract Background and purpose: Asthma is one of the most common chronic diseases in which inflammation plays an essential role in its pathophysiology. One of the secondary effects of asthma is depression, which is probably due to overlapping pathogenic mechanisms. One of the important mechanisms in the treatment of depression and asthma is to pay attention to removing inflammation and reducing oxidative stress. Purslane exerts its anti-inflammatory and antioxidant effects through NFqB and NOS pathways. This study aims to investigate the effect of the aqueous-alcoholic extract of the purslane plant on depression caused by experimental asthma using an Open Field Test and Forced Swimming Test in small laboratory mice.
Materials and methods: To investigate the aqueous-alcoholic extract of the purslane plant on depression caused by experimental asthma, 40 Syrian NMRI male mice were divided into 4 groups: control, asthmatic, and asthmatic receiving the extract at a dose of 50 mg/kg and 100 mg/kg. Syrian mice were injected and inhaled ovalbumin to develop asthma, and the control group received PBS solution in the same way. The treated groups received the extract at the same time as asthma induction.
Results: The results show that depression symptoms increased significantly after asthma induction. These symptoms were significantly reduced after the administration of purslane extract in a dose-dependent manner. The results indicated a significant increase in depression in the asthmatic group samples compared to the control group and also a significant decrease in depression in the groups treated with purslane extract compared to the asthmatic group.

Abstract Asthma is one of the most common non-communicable diseases characterized by reversible obstruction of airflow. It poses many problems for all age groups from infancy to old age. Various studies have shown that the occurrence of viral infections is associated with the severity of asthma symptoms so it can be prevented by controlling viral agents. In this study, the severity of the symptoms of persistent severe asthma with Haemophilus influenza infection was investigated. 31 patients with asthma with different degrees of disease were studied in this study. The results showed that in patients with asthma, the percentage of people with Haemophilus influenza was 71% and in 29% of other asthma cases, Haemophilus influenza virus was not observed. The relationship between asthma, cough and dyspnea with Haemophilus influenza infection showed that with increasing asthma symptoms, the severity of infection increases, and no significant association was observed between cough and dyspnea with Haemophilus influenza. Therefore, the results of this study clearly show that Haemophilus influenzae virus causes asthma symptoms to worsen in patients.

Abstract Background: A lot of patients are suffering from asthma. For decreasing the asthma symptoms, we studied the effects of conditioned medium (CM) of human amniotic membrane mesenchymal stem cells (hAM-MSCs) as a source of anti-inflammatory cytokines on splenocyte and lung tissue of asthmatic Balb/c mice.
Methods: Forty mice were categorized into four groups; ovalbumin (OVA)-induced asthma, CM-treated asthma, DMEM (Dulbecco's Modified Eagle Medium)-treated asthma, and saline control. Each group received related treatment. The lung alpha-smooth muscle actin (α-SMA)  and splenocyte inflammatory cytokines and IgE were examined through Western blot analysis.
Results: Western blot showed α-SMA overexpression in the OVA and DMEM groups compared with the saline group. CM therapy could significantly reverse it compared with OVA and OVA+DMEM categories by elevating IL-10 and IFN-γ and reducing IL-4, IgE, and TGF-β .
Conclusion: CM treatment could improve asthma symptoms by adjusting α-SMA in lung tissue and pro-inflammatory cytokines and IgE in splenocytes.