Personalized and Precision Medicine Journal

Abstract Numerous studies have been conducted to investigate the relationship between genetic factors and drug use tendency, many of which have shown a significant correlation between genetic factors and drug use, especially heroin and cocaine. The most important site of drug action is the brain, which contains a variety of nerve receptors. Dynorphins are opioid peptides derived from the prodynorphins precursor (PDYN). These opioid peptides are capable of binding to the three types of opioid receptors. Studies have shown that heroin and cocaine use is associated with increased PDYN gene expression in specific areas of the brain. In this study, we investigated the association between rs910080 polymorphism in the 3'UTR region and the number of VNTR sequences in the promoter region with the tendency of heroin use in 155heroin addicts and 150 control with RFLP method. Results showed a significant relationship between heroin abuse and CC genotype in rs910080 polymorphism (P = 0.001), but no significant relationship between VNTR promoter repeats and heroin abuse. Rs910080 polymorphism can be used as a distinguishing factor.

Abstract Diabetes Mellitus is defined as a metabolic disorder characterized by chronic hyperglycemia. The more prevalent form, type 2 diabetes, usually begins as insulin resistance, a disorder in which the cells do not use insulin properly. T2DM is a complex multifactorial disease in which multiple genetic variants interact with environmental factors to trigger the disease. There is sufficient evidence that T2DM has a strong genetic basis. One of the identified candidate genes associated with type 2 diabetes is CDKAL1, reduced expression of CDKAL1 would result in enhanced activity of CDK5 in β cells, which would lead to decreased insulin secretion. In this study, the association of polymorphism rs10946398 of CDKAL1 with type 2 diabetes was evaluated in 200 people by RFLP-PCR method. No significant association was found between the genotypes of polymorphism rs10946398 and type 2 diabetes in the target population. It is suggested that this polymorphism association with type 2 diabetes in larger populations evaluated.