Personalized and Precision Medicine Journal
Where Innovations Meets Personalized and Precision Medicine

Prevalence of Drug Resistance and some Pathogenic Factors in Uuropathogenic Escherichia coli (UPEC) Strains Isolated from Patients with Urinary Tract Infection

Document Type : Original Article

Authors

Zainab thaer Alshubidi 1
Ali Neamati 2
Massoud Homayoni 3

1 Master of Cellular and Molecular biology, Biology Department, Mashhad Unit, Islamic Azad University, Mashhad, Iran.

2 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

3 Assistant Professor of Biochemistry, Department of Biology, Mashhad Unit, Islamic Azad University, Mashhad, Iran

https://doi.org/10.22034/pmj.2024.713764
Abstract
Uropathogenic Escherichia coli is one of the most important causes of urinary tract infections. These strains possess various virulence factors, including adhesins, toxins, and iron acquisition systems. Virulence genes are situated on mobile genetic elements or in specific regions of the chromosome known as pathogenicity islands. In this study, 375 clinical samples from male and female patients suspected of having urinary tract infections were collected in the hospitals of Dhi Qar, Iraq, during the period from June 1, 2019, to November 1, 2019. Following the collection of 100 samples, bacterial isolation, DNA extraction, and antibiotic sensitivity tests were conducted using the disc diffusion method with the selected antibiotics. The presence of papC, aer, fimH, hly, cnf-1, and afa class genes was investigated using multiplex PCR. The results indicated that the highest frequency among the genes was associated with the fim gene (98%). The aer, papC, cnf-1, hly, and afa genes were also detected, with frequencies of 52%, 30%, 18%, 13%, and 11%, respectively. Additionally, the highest resistance and sensitivity among UPEC isolates were observed for amoxicillin (82.37%) and amikacin (92.35%) antibiotics, respectively.

Keywords

Escherichia coli
Virulence genes
Urinary tract infection
Antibiotic resistance
1. A. L. Flores-Mireles, J. N. Walker, M. Caparon, and S. J. Hultgren, "Urinary tract infections: epidemiology, mechanisms of infection and treatment options," Nature reviews microbiology, vol. 13, pp. 269-284, 2015.
2. T. M. Gibreel, Molecular Epidemiology of Uropathogenic Escherichia coli in North West England and characterisation of the ST131 clone in the region: The University of Manchester (United Kingdom), 2011.
3. P. Milart, E. Wozniakowska, S. Wozniak, T. Palacz, P. Czuczwar, W. Wrona, et al., "Urinary tract infections in the menopausal period: optimal management," Przeglad Menopauzalny, vol. 17, p. 23, 2013.
4.R. Stenutz, A. Weintraub, and G. Widmalm, "The structures of Escherichia coli O-polysaccharide antigens," FEMS microbiology reviews, vol. 30, pp. 382-403, 2006.
5. A. Jafari, M. Aslani, and S. Bouzari, "Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran," Iranian journal of microbiology, vol. 4, p. 102, 2012.
6.N. S. Merza and J. M. Jubrael, "The prevalence of virulence factors among Uropathogenic Escherichia coli strains isolated from different hospitals in Kurdistan region-Iraq," Int J Bioinform Biomed Eng, vol. 1, pp. 338-343, 2015.
7.Nazimi Ali, Naderi Mehsa, Jafarpour Mustafa, Miri Nargesi Mirsaed, & Sharifi Shahrashob. Frequency distribution of fimbria coding genes in E. coli isolated from patients with urinary tract infection (In Persian).
8.R. Polse, S. Yousif, and M. Assafi, "Prevalence and antimicrobial susceptibility patterns of uropathogenic E. coli among people in Zakho, Iraq," International Journal of Research in Medical Sciences, vol. 4, pp. 1219-1223, 2016.
9.M. M. AL-Nasrawi and A. B. AL-Hashimy, "COMPARISON OF VIRULENCE FACTORS PROFILE AND ANTIBIOTIC SENSITIVITY FOR ESCHERICHIA COLIBACTERIA ISOLATED FROM IRAQI WOMEN WITH URINARY TRACT INFECTIONS," Plant Archives, vol. 20, pp. 2225-2230, 2020.
 10. Amini, Muradali, Gholamali, & Shams Goli. (2017). Molecular identification of adhesion virulence, necrosis and hemolysin genes in UPEC and APEC strains isolated from clinical and poultry samples. Applied Biology Quarterly, 7(26), 35-42 (In Persian).
                         
Personalized and Precision Medicine Journal
Volume 9, Issue 33 - Serial Number 33
Original article
Spring 2024
Pages 7-13

  • Receive Date 27 January 2024
  • Revise Date 13 March 2024
  • Accept Date 20 May 2024

Home

Publication Ethics

Guide for Authors

Submit Manuscript

Contact Us