Where Innovations Meets Personalized and Precision Medicine

New FRET-Base Approach for Detection of HPV High Risk Genotype by DNA Capturing

Document Type : Original Article

Author

Postgraduate student of Genetic, Biology department, science faculty, Noor Danesh university, Meymeh, Isfahan, Iran

Abstract
Human Papillomavirus (HPV) is a highly prevalent virus responsible for several types of cancers, including cervical, throat, and anogenital cancers. Early detection and diagnosis are crucial for preventing the progression of HPV-related diseases. In this study, we introduce a new approach based on Förster Resonance Energy Transfer (FRET) method to identify viral DNA, was designed for the conserved region of the L1 gene sequence in high-risk genotypes 16, 18, 31 and 33. In order to create suitable temperature conditions for the attachment and also to identify the fluorescent signal, real time PCR device was used. The results of the specificity test showed 100% specificity and the limit of detection level of the method was reported to be 1000 copies/µl of the virus in the sample. The results of clinical sensitivity in the range of 86-96% between deferent genotype and the rate of false negative results was in the range of 14-22%. Based on this, it can be said that maybe the developed method cannot be proposed as a suitable alternative, but due to the response time and lower cost, it can be proposed as a quick screening method.

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Volume 10, Issue 39 - Serial Number 39
Original article
Autumn 2025
Pages 36-42

  • Receive Date 03 August 2025
  • Revise Date 10 October 2025
  • Accept Date 18 November 2025