Document Type : Original Article

Authors

1 Department of Medical genetic, national Institute for genetic Engineering and Biotechnology, Tehran, Iran

2 Faculty of Allied Health Sciences KuwaitnMedical Laboratory Sciences

3 Department of biology, Nourdanesh institute of higher education, Meymeh, Isfahan, Iran

4 Personalized Medicine Research Center of AmitisGen, Tehran, Iran

Abstract

The roles of miRNAs in autoimmunity are only beginning to be explored; they may be involved in regulating immune responses against self-tissues.miRNAs may contribute to disease progression and response to treatment in MS patients. Several studies on MS have analyzed the role or profile of miRNAs in different tissues including peripheral blood mononuclear cells (PBMCs).The current study evaluated mir-21 and mir-146a in CSF samples of patients with multiple sclerosis. Differential expression of the two miRNAs was detected in at least 80% of the CSF samples;however, additional functional studies and analyses of larger cohorts are needed to validate these results and to elucidate the real role of these miRNAs in the context of MS.
 

Keywords

1. Torre, L.A., Bray, F., Siegel, R.L., Ferlay, J., LortetTieulent, J., and
Jemal, A. (2015) Jemal Global cancer statistics, 2012. CA Cancer J.
Clin. 65,87108
2. Siegel, R.L., Miller, K.D., and Jemal, A. (2017) Cancer statistics,
2017. CA Cancer J. Clin. 67,730
3. Nieuwenhuis, L. and van den Brandt, P.A. (2019) Nut and peanut
butter consumption and the risk of lung cancer and its subtypes: a
prospective cohort study. Lung Cancer 128,5766
4. Filipowicz, W., Bhattacharyya, S.N., and Sonenberg, N. (2008)
Mechanisms of post-transcriptional regulation by microRNAs: are
the answers in sight? Nat. Rev. Genet. 9
5. Hu, Y., Yu, C.Y., Wang, J.L., Guan, J., Chen, H.Y., and Fang, J.Y.
(2014) MicroRNA sequence polymorphisms and the risk of different
types of cancer. Sci. Rep. 4, 3648
6. Esquela-Kerscher, A. and Slack, F.J. (2006) Oncomirs—
microRNAs with a role in cancer. Nat. Rev. Cancer 6, 259269
7. Zhong, J.H., Li, J., Liu, C.F., Liu, N., Bian, R.X., Zhao, S.M.,
Yan, S.Y., and Zhang, Y.B. (2017) Effects of microRNA-146a on the
proliferation and apoptosis of human osteoarthritis chondrocytes by
targeting TRAF6 through the NF-kB signaling pathway. Biosci. Rep.
28, 37
8. Hummel, R., Hussey, D.J., and Haier, J. (2010) MicroRNAs:
predictors and modifiers of chemo- and radiotherapy in different
tumour types. Eur. J. Cancer 46, 298311
9. Volpe, M.V., Pham, L., Lessin, M., Ralston, S.J., Bhan, I., Cutz, E.,
and Nielsen, H.C. (2003) Expression of Hoxb-5 during human lung
development and in congenital lung malformations. Birth Defects
Res. Part A Clin. Mol. Teratol. 67, 550556
10. Vinci, S., Gelmini, S., Pratesi, N., Conti, S., Malentacchi, F., Simi,
L., Pazzagli, M., and Orlando, C. (2011) Genetic variants in miR-
146a, miR-149, miR-196a2, miR-499 and their influence on relative
expression in lung cancers. Clin. Chem. Lab. Med. 49, 20732080
11. Xiao, S., Sun, S., Long, W., Kuang, S., Liu, Y., Huang, H.,
Zhou, J., Zhou, Y., and Lu, X. (2018) A meta-analytic review of the
association between two common SNPs in miRNAs and lung cancer
susceptibility. Onco Targets Ther. 11, 24192427
12. World Medical Association (2013) World Medical Association
Declaration of Helsinki: ethical principles for medical research
involving human subjects. JAMA, 310, 21912194
13. Sodhi, K.K., Bahl, C., Singh, N., Behera, D., and Sharma, S.
(2015) Functional genetic variants in premiR-146a and 196a2 genes
are associated with risk of lung cancer in North Indians. Future
Oncol. 11, 215921731
14. Hu, Z., Chen, J., Tian, T., Zhou, X., Gu, H., Xu, L., Zeng, Y., Miao,
R., Jin, G., Ma, H., Chen, Y., and Shen, H. (2008) Genetic variants of
miRNA sequences and non small cell lung cancer survival. J. Clin.
Invest. 118, 26002608
15. Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene
expression data using real time quantitative PCR and 2-ct method.
Methods 25, 402408
16. Shang, W., Pang, M., and Liu, Y. (2016) High resolution melting
analysis of the correlation of the miRNA-196a2 gene polymorphism
and lung cancer. BMU J. 2,8588
17. Jazdzewski K, Murray EL, Franssila K, Jarzab B, Schoenberg DR,de la Chapelle A. Common SNP in pre-miR-146a decreases mature
miR expression and predisposes to papillary thyroid carcinoma. Proc
Natl Acad Sci USA. 2008, 105:7269-7274.
18. Shen J, Ambrosone CB, DiCioccio RA, Odunsi K, Lele SB,
Zhao H. A functional polymorphism in the miR-146a gene and age
of familial breast/ovarian cancer diagnosis. Carcinogenesis. 2008,
29:1963-1966.
19.Okubo M, Tahara T, Shibata T, Yamashita H, Nakamura M,
Yoshioka D, Yonemura J, Kamiya Y, Ishizuka T, Nakagawa Y,
Nagasaka M, Iwata M, Yamada H, Hirata I, Arisawa T. Association
study of common genetic variants in pre-microRNAs in patients with
ulcerative colitis. J Clin Immunol. 2010, 31:69-73.
20. Xu T, Zhu Y, Wei QK, Yuan Y, Zhou F, Ge YY, Yang JR, Su H,
Zhuang SM. A functional polymorphism in the miR-146a gene is
associated with the risk for hepatocellular carcinoma. Carcinogenesis.
2008, 29:2126-2131.
21. Guo H, Wang K, Xiong G, Hu H, Wang D, Xu X, Guan X, Yang
K, Bai Y. A functional variant in microRNA-146a is associated withrisk of esophageal squamous cell carcinoma in Chinese Han. Fam
Cancer. 2010, 9:599-603.
22. Yue C, Wang M, Ding B, Wang W, Fu S, Zhou D, Zhang Z, Han S.
Polymorphism of the pre-miR-146a is associated with risk of cervical
cancer in a Chinese population. Gynecol Oncol. 2011, 122: 33-37.
23. Zeng Y, Sun QM, Liu NN, Dong GH, Chen J, Yang L, Wang B.
Correlation between pre-miR-146a C/G polymorphism and gastric
cancer risk in Chinese population. World J Gastroenterol. 2010,
16:3578-3583.
24. Xu B, Feng NH, Li PC, Tao J, Wu D, Zhang ZD, Tong N, Wang
JF, Song NH, Zhang W, Hua LX, Wu HF. A functional polymorphism
in Pre-miR-146a gene is associated with prostate cancer risk and
mature miR-146a expression in vivo. Prostate. 2010, 70:467-472.
25. Jeon HS, Lee YH, Lee SY, Jang JA, Choi YY, Yoo SS, Lee WK,
Choi JE, Son JW, Kang YM, Park JY. A common polymorphism in
pre-microRNA-146a is associated with lung cancer risk in a Korean
population. Gene. 2014, 534:66-71.